Detection and quantitation of lactoferrin in bovine whey samples by reversed-phase high-performance liquid chromatography on polystyrene-divinylbenzene

被引：56

作者：

Palmano, KP

Elgar, DF

机构：

[1] Massey Univ, Inst Fundamental Sci, Palmerston North, New Zealand

[2] New Zealand Dairy Res Inst, Palmerston, New Zealand

来源：

JOURNAL OF CHROMATOGRAPHY A | 2002年 / 947卷 / 02期

关键词：

whey; milk; food analysis; lactoferrin; whey proteins; proteins;

D O I：

10.1016/S0021-9673(01)01563-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An existing RP-HPLC method for the measurement of the major bovine whey proteins in bovine whey samples and powders has been extended to include the analysis of the minor bovine whey protein lactoferrin. Lactoferrin could be detected and quantitated at levels down to 0.2 mug and linear calibration was observed between 0.2 and 30 mug. Reliable quantitation of lactoferrin in whey samples could be achieved provided the bovine serum albumin to lactoferrin ratio did not exceed 10:1. Quantitative data obtained by the RP-HPLC method compared favourably with data obtained by Mono S analytical chromatography. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：307 / 311

页数：5

共 14 条

[1] INVITRO GROWTH-INHIBITION OF MASTITIS-CAUSING COLIFORM BACTERIA BY BOVINE APO-LACTOFERRIN AND REVERSAL OF INHIBITION BY CITRATE AND HIGH-CONCENTRATIONS OF APO-LACTOFERRIN [J].