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Level of M(IP)2C sphingolipid affects plant defensin sensitivity, oxidative stress resistance and chronological life-span in yeast

被引：50
作者：
Aerts, AM
François, IEJA
Bammens, L
Cammue, BPA
Smets, B
Winderickx, J
Accardo, S
De Vos, DE
Thevissen, K
机构：
[1] Katholieke Univ Leuven, Ctr Microbial & Plant Genet, B-3001 Heverlee, Belgium
[2] Katholieke Univ Leuven, Lab Funct Biol, B-3001 Heverlee, Belgium
[3] Katholieke Univ Leuven, Ctr Surface Chem & Catalysis, B-3001 Heverlee, Belgium
来源：
FEBS LETTERS | 2006年 / 580卷 / 07期
关键词：
sphingolipid; plant defensin; oxidative stress; chronological life-span;
D O I：
10.1016/j.febslet.2006.02.061
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The antifungal plant defensin DmAMP1 interacts with fungal sphingolipids of mannosyldiinositolphosphorlceramide (M(IP)(2)C) class. We screened a Saccharomyces cerevisiae transposon (Tn) mutant library against DmAMP1 and identified one DmAMP1-resistant mutant with the Tn inserted in the M(IP)(2)C biosynthesis gene IPT1 (DmTn11) and one DmAMP1-hypersensitive mutant with the Tn inserted in rDNA (HsTnII). However, tetrad analysis pointed to HsTnII as a spontaneous mutant. Apparently, membranes of DmTn11 lack M(IP)(2)C, whereas membranes of HsTnII have increased M(IP)2C levels. In addition, DmTn11 and HsTnII are characterized by increased and reduced oxidative stress resistance/chronological life-span (CL), respectively. A putative involvement of M(IP)(2)C in oxidative stress and CL in yeast is discussed. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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页码：1903 / 1907
页数：5
相关论文
共 25 条
[1]   Nicotinamide and PNC1 govern lifespan extension by calorie restriction in Saccharomyces cerevisiae [J].
Anderson, RM ;
Bitterman, KJ ;
Wood, JG ;
Medvedik, O ;
Sinclair, DA .
NATURE, 2003, 423 (6936) :181-185
[2]  
Ausubel FM., 1993, Current Protocols in Molecular Biology
[3]   Synthesis of mannose-(inositol-P)(2)-ceramide, the major sphingolipid in Saccharomyces cerevisiae, requires the IPT1 (YDR072c) gene [J].
Dickson, RC ;
Nagiec, EE ;
Wells, GB ;
Nagiec, MM ;
Lester, RL .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (47) :29620-29625
[4]   Chronological aging-independent replicative life span regulation by Msn2/Msn4 and Sod2 in Saccharomyces cerevisiae [J].
Fabrizio, P ;
Pletcher, SD ;
Minois, N ;
Vaupel, JW ;
Longo, VD .
FEBS LETTERS, 2004, 557 (1-3) :136-142
[5]   Regulation of longevity and stress resistance by Sch9 in yeast [J].
Fabrizio, P ;
Pozza, F ;
Pletcher, SD ;
Gendron, CM ;
Longo, VD .
SCIENCE, 2001, 292 (5515) :288-290
[6]   Isolation and characterization of Neurospora crassa mutants resistant to antifungal plant defensins [J].
Ferket, KKA ;
Levery, SB ;
Park, C ;
Cammue, BPA ;
Thevissen, K .
FUNGAL GENETICS AND BIOLOGY, 2003, 40 (02) :176-185
[7]   MnSOD overexpression extends the yeast chronological (G0) life span but acts independently of Sir2p histone deacetylase to shorten the replicative life span of dividing cells [J].
Harris, N ;
Costa, V ;
MacLean, M ;
Mollapour, M ;
Moradas-Ferreira, P ;
Piper, PW .
FREE RADICAL BIOLOGY AND MEDICINE, 2003, 34 (12) :1599-1606
[8]   Chronological aging leads to apoptosis in yeast [J].
Herker, E ;
Jungwirth, H ;
Lehmann, KA ;
Maldener, C ;
Fröhlich, KU ;
Wissing, S ;
Büttner, S ;
Fehr, M ;
Sigrist, S ;
Madeo, F .
JOURNAL OF CELL BIOLOGY, 2004, 164 (04) :501-507
[9]   Suppressor analysis points to the subtle role of the LAG1 ceramide synthase gene in determining yeast longevity [J].
Jiang, JC ;
Kirchman, PA ;
Allen, M ;
Jazwinski, SM .
EXPERIMENTAL GERONTOLOGY, 2004, 39 (07) :999-1009
[10]   A comparison of the aging and apoptotic transcriptome of Saccharomyces cerevisiae [J].
Laun, P ;
Ramachandran, L ;
Jarolim, S ;
Herker, E ;
Liang, P ;
Wang, JX ;
Weinberger, M ;
Burhans, DT ;
Suter, B ;
Madeo, F ;
Burhans, WC ;
Breitenbach, M .
FEMS YEAST RESEARCH, 2005, 5 (12) :1261-1272
123