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Cell surface display of human immunodeficiency virus type 1 gp120 on Escherichia coli by using ice nucleation protein

被引：40
作者：
Kwak, YD
Yoo, SK
Kim, EJ
机构：
[1] Yonsei Univ, Coll Engn, Dept Biotechnol, Seoul 120749, South Korea
[2] Yonsei Univ, Bioprod Res Ctr, Seoul 120749, South Korea
来源：
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY | 1999年 / 6卷 / 04期
关键词：
D O I：
10.1128/CDLI.6.4.499-503.1999
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
A new system designed for cell surface display of recombinant proteins on Escherichia coli has been evaluated for expression of eukaryotic viral proteins. Human immunodeficiency virus type 1 (HIV-1) gp120 was fused to the C terminus of ice nucleation protein (INP), an outer membrane protein of Pseudomonas syringae. Western blotting, immunofluorescence microscopy, fluorescence-activated cell-sorting analysis, whole-cell enzyme-linked immunosorbent assay, and ice nucleation activity assay confirmed the successful expression of HIV-I gp120 on the surface of Escherichia coli, This study shows that the INP system can be used for the expression of eukaryotic viral proteins. There is also a possibility that the INP system can be used as an AIDS diagnostic system, an oral vaccine delivery system, and an expression system for various heterologous higher-molecular-weight proteins.
引用
收藏
页码：499 / 503
页数：5
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