Evaluation of a novel PCR-based diagnostic assay for detection of Mycobacterium tuberculosis in sputum samples

被引：7

作者：

Maher, M ^{[1
]}

Glennon, M ^{[1
]}

Martinazzo, G ^{[1
]}

Turchetti, E ^{[1
]}

Marcolini, S ^{[1
]}

Smith, T ^{[1
]}

Dawson, MT ^{[1
]}

机构：

[1] RAGGIO ITALGENE SPA, POMEZIA, ITALY

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1996年 / 34卷 / 09期

关键词：

D O I：

10.1128/JCM.34.9.2307-2308.1996

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We report on a PCR-based assay we have developed for the detection of Mycobacterium tuberculosis in sputum samples. One hundred sputum specimens, which included 34 culture-positive and 66 culture-negative specimens, were evaluated with this system. Of the 34 culture-positive specimens, 31 were PCR positive, and 60 of the culture-negative specimens were PCR negative. An internal standard has been included in the assay system to monitor PCR inhibition and to confirm the reliability of the PCR assay.

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页码：2307 / 2308

页数：2

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