Double-labeled fluorescent probes for 5' nuclease assays: Purification and performance evaluation

被引：18

作者：

Rudert, WA ^{[1
]}

Braun, ER ^{[1
]}

Faas, SJ ^{[1
]}

Menon, R ^{[1
]}

JaquinsGerstl, A ^{[1
]}

Trucco, M ^{[1
]}

机构：

[1] UNIV PITTSBURGH,SCH MED,PITTSBURGH,PA

来源：

BIOTECHNIQUES | 1997年 / 22卷 / 06期

关键词：

D O I：

10.2144/97226rr02

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An inexpensive method for the purification and evaluation of user-synthesized or crude commercially prepared double-labeled fluorescent probes is presented. These probes exhibit the characteristics required for use in 5'-nuclease assays, including efficient reporter dye quenching, target specificity and susceptibility to cleavage by Taq DNA polymerase during PCR amplification. The method is suitable for research laboratories that wish to develop 5' nuclease assays for the detection of PCR-amplified target sequences to eliminate the requirement for agarose gels and to advance throughput.

引用

页码：1140 / 1145

页数：6

共 10 条

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