Separation and quantitation of the four stereoisomers of itraconazole in pharmaceutical formulations by electrokinetic chromatography

被引：15

作者：

Castro-Puyana, M ^{[1
]}

Crego, AL ^{[1
]}

Marina, ML ^{[1
]}

机构：

[1] Univ Alcala de Henares, Fac Quim, Dept Quim Analit, E-28871 Alcala De Henares, Madrid, Spain

来源：

ELECTROPHORESIS | 2006年 / 27卷 / 04期

关键词：

antifungals; chiral separation; cyclodextrin; electrokinetic chromatography; itraconazole;

D O I：

10.1002/elps.200500347

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The four stereoisomers of itraconazole were resolved for the first time by EKC using a CD as chiral selector. A study on the enantiomeric separation ability of different neutral CDs was carried out. Heptakis-2,3,6-tri-O-methyl-beta-CD was shown to provide the highest values for the enantiomeric resolution. The influence of some experimental conditions, such as pH, chiral selector concentration, and temperature, on the enantiomeric separation was also studied. The use of a 100 mM phosphate buffer (pH 2.5), 30 mM in heptakis-2,3,6-tri-O-methyl-beta-CD together with an applied voltage of 30 kV and a temperature of 20 degrees C enabled the separation of the enantiomers of itraconazole with high resolutions (R-s > 3.0). Finally, the method was validated and successfully applied to the quantitation of itraconazole in three pharmaceutical formulations.

引用

页码：887 / 895

页数：9

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