Intestinal Microbiota in Healthy US Young Children and Adults-A High Throughput Microarray Analysis

被引:151
作者
Ringel-Kulka, Tamar [1 ]
Cheng, Jing [2 ]
Ringel, Yehuda [3 ]
Salojarvi, Jarkko [2 ]
Carroll, Ian [3 ]
Palva, Airi [2 ]
de Vos, Willem M. [2 ,4 ,5 ]
Satokari, Reetta [2 ]
机构
[1] Univ N Carolina, Dept Maternal & Child Hlth, Gillings Sch Global Publ Hlth, Chapel Hill, NC 27515 USA
[2] Univ Helsinki, Dept Vet Biosci, Div Microbiol & Epidemiol, Helsinki, Finland
[3] Univ N Carolina, Dept Med, Div Gastroenterol & Hepatol, Chapel Hill, NC USA
[4] Univ Helsinki, Haartman Inst, Helsinki, Finland
[5] Wageningen Univ, Microbiol Lab, NL-6700 AP Wageningen, Netherlands
基金
美国国家卫生研究院; 芬兰科学院;
关键词
IRRITABLE-BOWEL-SYNDROME; REAL-TIME PCR; HUMAN GASTROINTESTINAL-TRACT; FECAL MICROBIOTA; GUT MICROBIOTA; REDUCED DIVERSITY; ALLERGIC DISEASE; INCREASED RISK; ANTIBIOTIC USE; DIARRHEA;
D O I
10.1371/journal.pone.0064315
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];
摘要
It is generally believed that the infant's microbiota is established during the first 1-2 years of life. However, there is scarce data on its characterization and its comparison to the adult-like microbiota in consecutive years. Aim: To characterize and compare the intestinal microbiota in healthy young children (1-4 years) and healthy adults from the North Carolina region in the U. S. using high-throughput bacterial phylogenetic microarray analysis. Methods: Detailed characterization and comparison of the intestinal microbiota of healthy children aged 1-4 years old (n = 28) and healthy adults of 21-60 years (n = 23) was carried out using the Human Intestinal Tract Chip (HITChip) phylogenetic microarray targeting the V1 and V6 regions of 16S rRNA and quantitative PCR. Results: The HITChip microarray data indicate that Actinobacteria, Bacilli, Clostridium cluster IV and Bacteroidetes are the predominant phylum-like groups that exhibit differences between young children and adults. The phylum-like group Clostridium cluster XIVa was equally predominant in young children and adults and is thus considered to be established at an early age. The genus-like level show significant 3.6 fold (higher or lower) differences in the abundance of 26 genera between young children and adults. Young U. S. children have a significantly 3.5-fold higher abundance of Bifidobacterium species than the adults from the same location. However, the microbiota of young children is less diverse than that of adults. Conclusions: We show that the establishment of an adult-like intestinal microbiota occurs at a later age than previously reported. Characterizing the microbiota and its development in the early years of life may help identify 'windows of opportunity' for interventional strategies that may promote health and prevent or mitigate disease processes.
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页数:10
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