Short-term ozone exposure upregulates P-selectin in normal human airways

被引:26
作者
Krishna, MT
Blomberg, A
Biscione, GL
Kelly, F
Sandstrom, T
Frew, A
Holgate, S
机构
[1] ST THOMAS HOSP, RAYNE INST, LONDON SE1 7EH, ENGLAND
[2] UNIV HOSP N SWEDEN, DEPT PULM MED & ALLERGOL, UMEA, SWEDEN
[3] NATL INST WORKING LIFE, DIV MED, UMEA, SWEDEN
关键词
D O I
10.1164/ajrccm.155.5.9154895
中图分类号
R4 [临床医学];
学科分类号
1002 ; 100602 ;
摘要
Short-term exposure to ambient levels of ozone induces neutrophilic bronchitis. To investigate the early events contributing to inflammatory cell recruitment in the airways we exposed 12 healthy nonsmoking volunteers to 0.12 ppm ozone or filtered air for 2 h on two separate occasions. Spirometry and fiberoptic bronchoscopy were performed immediately and at 1.5 h after the two exposures, respectively. Total protein, albumin, and total and differential cell counts were performed on the bronchial wash and BAL fluid. Bronchial biopsies were embedded in glycol methacrylate and immunostained for inflammatory cells, including neutrophils, mast cells, total T-cells (CD3), T-cell subset CD8, and leukocyte endothelial adhesion molecules, including VCAM-1, ICAM-I, E-selectin, and P-selectin. No significant changes were observed in FEV1, FVC, or any inflammatory indices in the bronchial wash and BAL fluid. In addition, no significant differences were seen in inflammatory cell numbers or percentages of vessels expressing VCAM-1, E-selectin, or ICAM-1 in the biopsies. The percentage of vessels expressing P-selectin increased significantly after ozone exposure: p = 0.016; median (IQR), 28.76 (26.36-36.94) versus 47.06 (38.14-56.86)%. The upregulation of P-selectin could signify an early inflammatory response to ozone such as margination and rolling of the neutrophils on the vessel wall prior to transendothelial migration.
引用
收藏
页码:1798 / 1803
页数:6
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