Putative serine/threonine protein kinase expressed in complement-resistant forms of Entamoeba histolytica

被引：18

作者：

Urban, B ^{[1
]}

Blasig, C ^{[1
]}

Forster, B ^{[1
]}

Hamelmann, C ^{[1
]}

Horstmann, RD ^{[1
]}

机构：

[1] BERNHARD NOCHT INST TROP MED,D-20359 HAMBURG,GERMANY

来源：

MOLECULAR AND BIOCHEMICAL PARASITOLOGY | 1996年 / 80卷 / 02期

关键词：

amoebiasis; E-histolytica; subtractive hybridization; subtractive cDNA library; alternate polyadenylation;

D O I：

10.1016/0166-6851(96)02684-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Entamoeba histolytica is susceptible to complement attack in its lumen-dwelling state and develops complement resistance during pathogenic tissue invasion. As experimental evidence suggests that this change in phenotype is accompanied by a change in gene expression, we constructed a subtractive cDNA library to identify genes involved. Poly(A)(+)RNA from complement-sensitive trophozoites was subtracted from single stranded cDNA derived from complement-resistant ones. Transcripts enriched in the library were found to code for a putative polypeptide comprising all sequence elements characteristic for serine/threonine protein kinases. The gene contains an intron of 46 nucleotides and two polyadenylation sites. Northern-blot analyses confirmed that the gene is expressed in both tissue-derived and laboratory-grown forms of complement-resistant E. histolytica.

引用

页码：171 / 178

页数：8

共 33 条

[1] IMMUNOLOGICAL SIMILARITY BETWEEN THE 170 KD AMEBIC ADHERENCE GLYCOPROTEIN AND HUMAN BETA-2 INTEGRINS [J].