Preparation of sterol-imprinted polymers with the use of 2-(methacryloyloxy)ethyl phosphate

被引：32

作者：

Kugimiya, A ^{[1
]}

Kuwada, Y ^{[1
]}

Takeuchi, T ^{[1
]}

机构：

[1] Hiroshima City Univ, Fac Informat Sci, Lab Synthet Biochem, Asaminami Ku, Hiroshima 7313194, Japan

来源：

JOURNAL OF CHROMATOGRAPHY A | 2001年 / 938卷 / 1-2期

关键词：

molecular imprinting; sterols; methacryloyloxyethyl phosphate; phosphates; cholesterol;

D O I：

10.1016/S0021-9673(01)01384-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Steroid-selective polymers were prepared by the molecular imprinting technique, using 2-(methacryloyloxy)ethyl phosphate as functional monomer. The retentivity and selectivity of the obtained imprinted polymers were evaluated by liquid chromatography. The cholesterol-imprinted polymer showed higher affinity for cholesterol than that for cholesterol derivatives. The selectivity of the imprinted polymer was superior to the imprinted polymer prepared with the conventional functional monomer, 2-(trifluoromethyl)acrylic acid. Estradiol was also imprinted and gave similar results, demonstrating that 2-(methacryloyloxy)ethyl phosphate would be suitable for imprinted polymers of cholesterol and related compounds. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：131 / 135

页数：5

共 14 条

[1] BINDING OF SH2 DOMAINS OF PHOSPHOLIPASE-C-GAMMA-1, GAP, AND SRC TO ACTIVATED GROWTH-FACTOR RECEPTORS [J].