A novel specific DNA marker in Saccharomyces bayanus for species identification of the Saccharomyces sensu stilcto complex

被引：6

作者：

Huang, Chien-Hsun ^{[1
]}

Lee, Fwu-Ling ^{[1
]}

Tai, Chun-Ju ^{[1
]}

机构：

[1] Food Ind Res & Dev Inst, Bioresource Collect & Res Ctr, Hsinchu 30099, Taiwan

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2008年 / 75卷 / 03期

关键词：

DNA fingerprinting; Novel DNA sequence; Soccharomyces bayanus; Saccharomyces sensu stricto complex; Species identification;

D O I：

10.1016/j.mimet.2008.08.005

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This study was based on RAPD fingerprinting for species identification of the Saccharomyces sensu stricto complex. 40 random primers were used for RAPD analysis. The results showed that one of these primers, OPT-18, produced a 974 bp species-specific band, which was only found in the tested S. bayanus. Afterward this specific fragment was isolated from agarose gel and ligated into vector for DNA sequencing. A pair of primer SpeOPT18Sbay-F2 and SpeOPT18Sbay-R2 were designed according to the cloned species-specific sequence, which was employed for PCR with the template DNA of the S. sensu stricto strains, single 779 bp species-specific band was only found in S. bayanus. Therefore, we conclude that our novel species DNA marker could be used to rapidly and accurately identify the species of S. bayanus from S. sensu stricto complex by direct PCR. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：531 / 534

页数：4

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