Resolving isomeric peptide mixtures: A combined HPLC/ion mobility-TOFMS analysis of a 4000-component combinatorial library

被引：80

作者：

Barnes, CAS ^{[1
]}

Hilderbrand, AE

Valentine, SJ

Clemmer, DE

机构：

[1] Indiana Univ, Dept Chem, Bloomington, IN 47405 USA

[2] Beyond Genom, Waltham, MA 02451 USA

来源：

ANALYTICAL CHEMISTRY | 2002年 / 74卷 / 01期

关键词：

D O I：

10.1021/ac0108562

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A reversed-phase high-performance liquid chromatography (HPLC) separation approach has been combined with ion mobility/time-of-flight (TOF) mass spectrometry in order to characterize a combinatorial peptide library designed to contain 4000 peptides of the general form NH2-Xxx-Xxx-Xxx-CO2H, NH2-Ala-Xxx-Xxx-Xxx-CO2H, NH2-Ser-Ala-Xxx-Xxx-Xxx-CO2H and NH2-Leu-Ser-Ala-Xxx-Xxx-Xxx-CO2H (where Xxx represents a randomization over 10 different amino acids: Ala, Arg, Asp, Glu, Gly, Leu, Lys, Phe, Ser, and Val). Addition of the gas-phase mobility separation between the HPLC separation and TOF measurement dimensions makes it possible to resolve many peptide isomers that have identical retention times (and masses).

引用

页码：26 / 36

页数：11

共 34 条

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