A statistical approach to optimization of fermentative production of poly(γ-glutamic acid) from Bacillus licheniformis NCIM 2324

被引：68

作者：

Bajaj, I. B. ^{[1
]}

Lele, S. S. ^{[1
]}

Singhal, R. S. ^{[1
]}

机构：

[1] Univ Bombay, Inst Chem Technol, Food Engn & Technol Dept, Bombay 400019, Maharashtra, India

来源：

BIORESOURCE TECHNOLOGY | 2009年 / 100卷 / 02期

关键词：

Poly(gamma-glutamic acid); Bacillus licheniformis; Optimization; Plackett-Burman design; Response surface methodology; GAMMA-GLUTAMIC ACID; POLY(L-GLUTAMIC ACID); AMMONIUM-SULFATE; CITRIC-ACID; BIOSYNTHESIS; GLYCEROL;

D O I：

10.1016/j.biortech.2008.06.047

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

This paper reports on the optimization of poly(gamma-glutamic acid) (PGA) production by Bacillus licheniformis NCIM 2324 using a statistical approach. One-factor-at-a-time method was used to investigate the effect of carbon sources, nitrogen sources and pH on PGA production. Plackett-Burman design was adopted to select the most important nutrients influencing the yield of PGA. After identifying effective nutrients, response surface methodology was used to develop a mathematical model to identify the optimum concentrations of the key nutrients for higher PGA production, and confirm its validity experimentally. PGA production increased significantly from 5.27 to 26.12 g/l when the strain was cultivated in the optimal medium developed by using statistical approach, as compared to basal medium. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：826 / 832

页数：7

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