Platycodi Radix attenuates dimethylnitrosamine-induced liver fibrosis in rats by inducing Nrf2-mediated antioxidant enzymes

被引：28

作者：

Choi, Jae Ho ^{[1
]}

Jin, Sun Woo ^{[1
]}

Kim, Hyung Gyun ^{[1
]}

Khanal, Tilak ^{[1
]}

Hwang, Yong Pil ^{[1
,2
]}

Lee, Kyung Jin ^{[3
]}

Choi, Chul Yung ^{[4
]}

Chung, Young Chul ^{[5
]}

Lee, Young Chun ^{[5
,6
]}

Jeong, Hye Gwang ^{[1
]}

机构：

[1] Chungnam Natl Univ, Coll Pharm, Dept Toxicol, Taejon, South Korea

[2] Int Univ Korea, Dept Pharmaceut Engn, Jinju, South Korea

[3] Asan Med Ctr, Asan Inst Life Sci, Seoul, South Korea

[4] Jeollanamdo Inst Nat Resources Res, Jeollanamdo, South Korea

[5] Int Univ Korea, Dept Food Sci, Jinju, South Korea

[6] Jangsaeng Doraji Co Ltd, Jinju, South Korea

来源：

FOOD AND CHEMICAL TOXICOLOGY | 2013年 / 56卷

基金：

新加坡国家研究基金会;

关键词：

Platycodi Radix; Liver fibrosis; Antioxidant enzymes; Nrf2; COX-2; TRANSCRIPTION FACTOR NRF2; HEPATIC-FIBROSIS; OXIDATIVE STRESS; AQUEOUS EXTRACT; NUCLEAR TRANSLOCATION; STELLATE CELL; GRANDIFLORUM; ACTIVATION; EXPRESSION; INDUCTION;

D O I：

10.1016/j.fct.2013.02.033

中图分类号：

TS2 [食品工业];

学科分类号：

100403 [营养与食品卫生学];

摘要：

The purpose of this study was to investigate the anti-fibrotic effects of the aqueous extract of the Platycodi Radix root (Changkil: CK) on dimethylnitrosamine (DMN)-induced liver fibrosis in rats. DMN treatment for 4 weeks led to marked liver fibrosis as assessed by serum biochemistry, histopathological examination, and hepatic lipid peroxidation and collagen content. CK significantly inhibited DMN-induced increases in serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities, fibrosis score, and hepatic malondialdehyde and collagen content. CK also inhibited DMN-induced reductions in rat body and liver weights. Reverse transcription polymerase chain reaction (RT-PCR) and western blot analyses revealed that CK inhibited DMN-induced increases in matrix metalloproteinase-13 (MMP-13), tissue inhibitor of metalloproteinase-1 (TIMP-1), and tumor necrosis factor-alpha (TNF-alpha) mRNA, and collagen type I and alpha-smooth muscle actin protein. DMN-induced cyclooxygenase-2 (COX-2) expression and nuclear factor-kappa B (NF-kappa B) activation was reduced by CK treatment. Furthermore, CK induced activation of nuclear erythroid 2-related factor 2 (Nrf2)-mediated antioxidant enzymes such as gamma-glutamylcysteine synthetase (gamma-GCS), heme oxygenase-1 (HO-1), NAD(P)H quinone oxidoreductase 1 (NQO1), and glutathione-S-transferase (GST) in HepG2 cells. These results demonstrated that CK attenuates DMN-induced liver fibrosis through the activation of Nrf2-mediated antioxidant enzymes. (C) 2013 Elsevier Ltd. All rights reserved.

引用

页码：231 / 239

页数：9

共 49 条

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