Protective effect of wogonin on proinflammatory cytokine generation via Jak1/3-STAT1/3 pathway in lipopolysaccharide stimulated BV2 microglial cells

被引：58

作者：

Yeh, Chung-Hsin ^{[1
]}

Shih, Hung-Che ^{[2
,3
]}

Hong, Hui-Mei ^{[4
]}

Lee, Shiuan-Shinn ^{[5
]}

Yang, Ming-Ling ^{[6
]}

Chen, Chun-Jung ^{[7
,8
]}

Kuan, Yu-Hsiang ^{[2
,3
]}

机构：

[1] Show Chwan Mem Hosp, Dept Neurol, Changhua, Taiwan

[2] Chung Shan Med Univ, Sch Med, Dept Pharmacol, Taichung 402, Taiwan

[3] Chung Shan Med Univ Hosp, Dept Pharm, Taichung, Taiwan

[4] Chung Shan Med Univ, Sch Biomed Sci, Taichung, Taiwan

[5] Chung Shan Med Univ, Sch Publ Hlth, Taichung, Taiwan

[6] Chung Shan Med Univ, Sch Med, Dept Anat, Taichung, Taiwan

[7] Taichung Vet Gen Hosp, Dept Educ & Res, Taichung, Taiwan

[8] Tunghai Univ, Ctr Gen Educ, Taichung 40704, Taiwan

来源：

TOXICOLOGY AND INDUSTRIAL HEALTH | 2015年 / 31卷 / 10期

关键词：

Wogonin; BV2 microglial cells; STAT; Jak; antiinflammation; A-GLYCIDYL-METHACRYLATE; NECROSIS-FACTOR-ALPHA; ACUTE LUNG INJURY; SIGNAL-TRANSDUCTION; MACROPHAGES; ACTIVATION; STATS; PHOSPHORYLATION; SUPPRESSION; INHIBITION;

D O I：

10.1177/0748233713485886

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

100235 [预防医学];

摘要：

Wogonin is a flavonoid compound which exhibits antioxidation, anti-inflammation, neuroprotection, and antitumorgenesis functions. However, the mechanism of how wogonin reduces proinflammatory cytokine generation in activated microglia is unclear. At present, we found wogonin inhibited lipopolysaccharide (LPS)-/interferon- (INF-)-induced generation of interleukin-6 (IL-6) and tumor necrosis factor- (TNF-). Wogonin exhibited parallel inhibition on LPS-/INF--induced expression of IL-6 and TNF- messenger RNA at the same concentration range. LPS-/INF--induced phosphorylation of signal transduction and transcription 1 and 3 (STAT1/3) were also inhibited by wogonin. Although wogonin expressed only weak inhibitory effect on LPS-/INF--induced phosphorylation of Janus kinase-2 (Jak-2) and tyrosine kinase (Tyk)-2, it significantly attenuated the phosphorylation of Jak-1 and Jak-3. These results indicated that the blockade of IL-6 and TNF- production by wogonin in LPS-/INF--stimulated BV2 microglial cells was attributed mainly to the interference in Jak-1/-3-STAT1/3 signaling pathway.

引用

页码：960 / 966

页数：7

共 32 条

[1]

Review: Axon pathology in age-related neurodegenerative disorders [J].