Reverse transcription loop-mediated isothermal amplification for the detection of highly pathogenic porcine reproductive and respiratory syndrome virus

被引：36

作者：

Chen, Hao-tai ^{[1
]}

Zhang, Jie ^{[1
]}

Sun, De-hui ^{[1
]}

Ma, Li-na ^{[1
]}

Liu, Xiang-tao ^{[1
]}

Quan, Kai ^{[2
]}

Liu, Yong-sheng ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, Lanzhou Vet Res Inst, State Key Lab Vet Etiol Biol, Key Lab Anim Virol,Minist Agr,Dept Virol, Lanzhou 730046, Gansu, Peoples R China

[2] Zhengzhou Coll Anim Husb Engn, Henan Prov Key Lab Anim Immunol, Zhengzhou, Peoples R China

来源：

JOURNAL OF VIROLOGICAL METHODS | 2008年 / 153卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Porcine reproductive and respiratory; syndrome virus (PRRSV); Highly pathogenic; Detection; Reverse transcription loop-mediated; isothermal amplification (RT-LAMP);

D O I：

10.1016/j.jviromet.2008.07.006

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay targeting the open reading frames la of highly pathogenic porcine reproductive and respiratory syndrome virus genome was developed. The 10 reference strains, I clinical isolation strain and 122 positive samples were tested. Positive reactions were confirmed for all strains and specimens by reverse transcription loop-mediated isothermal amplification and nested reverse transcription polymerase chain reaction (RT-PCR). The results showed this detection technique is more reliable and convenient for rapid and sensitive diagnosis of highly pathogenic porcine reproductive and respiratory syndrome virus infection. (C) 2008 Elsevier B.V. All rights reserved.

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收藏

页码：266 / 268

页数：3

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