Rapid identification of Gram-negative organisms from blood culture bottles using a modified extraction method and MALDI-TOF mass spectrometry

被引：32

作者：

Gray, Timothy J. ^{[1
]}

Thomas, Lee ^{[1
]}

Olma, Tom ^{[1
]}

Iredell, Jonathan R. ^{[1
,2
,3
]}

Chen, Sharon C. -A. ^{[1
,3
]}

机构：

[1] Westmead Hosp, Ctr Infect Dis & Microbiol Lab Serv, Inst Clin Pathol & Med Res, Westmead, NSW 2145, Australia

[2] Westmead Hosp, Ctr Res Excellence Crit Infect, Westmead Millennium Inst, Westmead, NSW 2145, Australia

[3] Univ Sydney, Westmead Hosp, Sydney Emerging Infect Dis Inst, Westmead, NSW 2145, Australia

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2013年 / 77卷 / 02期

关键词：

Gram negative bacilli; Blood culture; Bloodstream infection; MALDI-TOF; Mass-spectrometry; DESORPTION IONIZATION-TIME; BACTERIA;

D O I：

10.1016/j.diagmicrobio.2013.06.016

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

The application of matrix-assisted laser desorption/ionisation time-of-flight (MALDI-TOF) mass spectrometry (MS) directly to blood culture broth has potential to identify bloodstream infection earlier and facilitate timely management. We prospectively tested a novel, rapid, and inexpensive in-house spin-lysis protocol with formic acid extraction and compared MALDI-TOF MS identification of Gram-negative bacteria with traditional phenotypic methods (Phoenix (TM)) directly from 318 BACTEC (TM) (Becton Dickinson, Franklin Lakes, USA) blood cultures. The MS score was >= 1.7 in 268 (91.8%) monomicrobial broths, with concordance to genus and species level of 100% and 97.0%, respectively. MALDI-TOF MS still has limited capacity to detect all species in polymicrobial broths. Crown Copyright (C) 2013 Published by Elsevier Inc. All rights reserved.

引用

页码：110 / 112

页数：3

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