Chromosome 7-Centric Analysis of Proteomics Data from a Panel of Human Colon Carcinoma Cell Lines

被引：7

作者：

Fanayan, Susan ^{[1
]}

Smith, Joshua T. ^{[2
,3
]}

Sethi, Manveen K. ^{[1
]}

Cantor, David ^{[1
]}

Goode, Robert ^{[4
]}

Simpson, Richard J. ^{[5
]}

Baker, Mark S. ^{[1
]}

Hancock, William S. ^{[1
,2
,3
]}

Nice, Edouard ^{[4
]}

机构：

[1] Macquarie Univ, Dept Chem & Biomol Sci, Sydney, NSW 2109, Australia

[2] Northeastern Univ, Barnett Inst, Boston, MA 02115 USA

[3] Northeastern Univ, Dept Chem & Chem Biol, Boston, MA 02115 USA

[4] Monash Univ, Dept Biochem & Mol Biol, Clayton, Vic 3800, Australia

[5] La Trobe Univ, La Trobe Inst Mol Sci, Bundoora, Vic 3086, Australia

来源：

JOURNAL OF PROTEOME RESEARCH | 2013年 / 12卷 / 01期

基金：

澳大利亚国家健康与医学研究理事会;

关键词：

chromosome; 7; human proteome project; colon cancer; mass spectrometry; MASS-SPECTROMETRY; DNA-SEQUENCE; GROWTH; CANCER; PROTEINS; CHROMATOGRAPHY; GLYCOPROTEINS; ESTABLISHMENT; ABUNDANCE; TANDEM;

D O I：

10.1021/pr300906y

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In this manuscript, we describe a shotgun proteomics approach for a comprehensive proteomic analysis of samples including total lysates, membrane, secretome, and exosome fractions from a panel of colorectal cancer cell lines. We will present an analysis of our proteomics data in two alternative formats. First we will discuss a traditional analysis of our data, in which we identify a number of cancer-associated proteins using various proteomic data analysis tools. In a second approach, we use a chromosome format to organize the proteomic data on chromosome 7, allowing the identification of clusters of cancer-associated genes with boundaries defined by physical proximity on different chromosomes.

引用

页码：89 / 96

页数：8

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