Improved Ruthenium II tris (bathophenantroline disulfonate) staining and destaining protocol for a better signal-to-background ratio and improved baseline resolution

被引：103

作者：

Lamanda, A

Zahn, A

Röder, D

Langen, H

机构：

[1] Univ Bern, Dept Chem & Biochem, CH-3012 Bern, Switzerland

[2] F Hoffmann La Roche, Basel, Switzerland

来源：

PROTEOMICS | 2004年 / 4卷 / 03期

关键词：

baseline resolution; destaining; ruthenium II tris (bathophenantroline disulfonate); signal-to-background;

D O I：

10.1002/pmic.200300587

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In proteomics the ability to visualize proteins from electropherograms is essential. Here a new protocol for staining and destaining gels treated with Ruthenium II tris (bathophenantroline disulfonate) is presented. The method is compared with the silver-staining procedure of Swain and Ross, the Ruthenium II tris (bathophenantroline disulfonate) stain described by Rabilloud (Rabilloud T., Strub, S. M. Luche, S., Girardet, S. L. et al., Proteomics 2001, 1, 699-704) and the SYPRO Ruby gel stain. The method offers a better signal-to-background ratio with improved baseline resolution for both sodium dodecyl sulfate-polyacrylamide gels and two-dimensional gels.

引用

页码：599 / 608

页数：10

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