Leucocyte and platelet adhesion in different layers of the small bowel during experimental total warm ischaemia and reperfusion

被引:20
作者
Beuk, R. J. [1 ]
Tangelder, G. -J. [2 ]
Maassen, R. L. J. G. [3 ]
Quaedackers, J. S. L. T. [3 ]
Heineman, E. [3 ]
Egbrink, M. G. A. Oude [4 ]
机构
[1] Med Spectrum Twente, Dept Surg, NL-7500 KA Enschede, Netherlands
[2] Vrije Univ Amsterdam, Inst Cardiovasc Res, Physiol Lab, Amsterdam, Netherlands
[3] Univ Hosp Maastricht, Cardiovasc Res Inst Maastricht, Dept Surg, Maastricht, Netherlands
[4] Univ Hosp Maastricht, Cardiovasc Res Inst Maastricht, Dept Physiol, Maastricht, Netherlands
关键词
D O I
10.1002/bjs.6320
中图分类号
R61 [外科手术学];
学科分类号
摘要
Background: Ischaemia and reperfusion (IR) of the small bowel is involved in many clinical conditions. A key component in IR-induced tissue damage is microvascular dysfunction. The aim was to investigate the role of leucocytes and platelets in capillary flow impediment and tissue damage. Methods: Anaesthetized rats were subjected to 30 min warm ischaemia of the small bowel, followed by 1 h reperfusion. To elucidate the influence of leucocytes on platelet adhesion, leucocyte-vessel wall interactions induced by IR were prevented by anti-platelet activating factor (PAF) or anti-intercellular adhesion molecule (ICAM)-1. Intravital videomicroscopy was performed and tissue injury was evaluated histologically. Results: In submucosal venules, IR induced an increase in the median number of interacting leucocytes from 3 to 10 and 20 leucocytes per 100-mu m venule segment after 10 and 60 min reperfusion respectively. Anti-PAF or anti-ICAM-1 completely attenuated this increase, resulting in an eightfold improvement in submucosal capillary flow and reduced tissue injury. Shedding of villi no longer occurred. Platelet-vessel wall interactions occurred particularly in submucosal venules, but were not affected by anti-PAF or anti-ICAM-1. Conclusion: Small bowel IR initiated all inflammatory, and thrombotic response in the submucosal layer only. Attenuation of leucocyte adhesion improved submucosal capillary perfusion, preventing shedding of mucosal villi.
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页码:1294 / 1304
页数:11
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