Characterization of microsatellite loci in the endangered St. Vincent Parrot, Amazona guildingii

被引：35

作者：

Russello, M

Calcagnotto, D

DeSalle, R

Amato, G

机构：

[1] Amer Museum Nat Hist, Mol Systemat Lab, New York, NY 10024 USA

[2] Columbia Univ, Ctr Environm Res & Conservat, New York, NY USA

[3] Wildlife Conservat Soc, Sci Resource Ctr, Bronx, NY USA

来源：

MOLECULAR ECOLOGY NOTES | 2001年 / 1卷 / 03期

关键词：

dinucleotide; microsatellites; Neotropical; polymorphic; primers; Psittacidae;

D O I：

10.1046/j.1471-8278.2001.00061.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Microsatellite loci were isolated within the St Vincent parrot (Amazona guildingii), an endangered island endemic, from a GT(n) enriched genomic library. Nine polymorphic loci were characterized varying from two to nine alleles per locus. Seven loci exhibited levels of heterozygosity ranging from 39 to 100% within the 28 individuals genotyped. Furthermore, the primers were demonstrated to cross-amplify within four genera of Neotropical parrots, indicating the potential utility of these markers to further population-level studies and conservation efforts of New World Psittacines.

引用

页码：162 / 164

页数：3

共 6 条

[1]

[Anonymous], 1998, Primer 3

[2] Microsatellite loci isolated from Odontesthes argentinensis and the O-perugiae species group and their use in other South American silverside fish [J].