ANALYSIS OF NONFUNCTIONAL RIBOSOMAL RNA DECAY IN SACCHAROMYCES CEREVISIAE

被引:6
作者
Cole, Sarah E. [1 ]
LaRiviere, Frederick J. [2 ]
机构
[1] Brandeis Univ, Dept Biol, Waltham, MA 02254 USA
[2] Washington & Lee Univ, Dept Chem, Lexington, VA 24450 USA
来源
RNA TURNOVER IN EUKARYOTES: ANALYSIS OF SPECIALIZED AND QUALITY CONTROL RNA DECAY PATHWAYS | 2008年 / 449卷
关键词
D O I
10.1016/S0076-6879(08)02412-9
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Mature rRNA are normally extremely stable in rapidly growing cells. However, studies show that some mature rRNA in Saccharomyces cerevisiae are, in fact, turned over quite rapidly by the nonfunctional rRNA decay (NRD) pathway. NRD eliminates the RNA component of mature but defective ribosomal subunits and ribosomes. NRD was discovered using rDNA reporter plasmids to express and track the fate of rRNA containing mutations in functionally important regions of the ribosome. This chapter outlines some of the available rDNA reporter plasmids that can be used to study NRD and describes assays to test for functionality and stability of rRNA in yeast.
引用
收藏
页码:239 / 259
页数:21
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