Identification and characterization of a fish natural resistance-associated macrophage protein (NRAMP) cDNA

被引：28

作者：

Saeij, JPJ ^{[1
]}

Wiegertjes, GF ^{[1
]}

Stet, RJM ^{[1
]}

机构：

[1] Wageningen Univ Agr, Wageningen Inst Anim Sci, Cell Biol & Immunol Grp, NL-6700 AH Wageningen, Netherlands

来源：

IMMUNOGENETICS | 1999年 / 50卷 / 1-2期

关键词：

NRAMP; fish; carp; evolution; expression;

D O I：

10.1007/s002510050686

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The mouse Lsh/lty/Bcg locus regulates natural resistance to intracellular pathogens, and the Nrampl gene was isolated as its candidate. Nramp is part of a small family of at least two genes, Nramp1 and Nramp2. In the present study, a full-length cDNA for carp NRAMP has been isolated and characterized. Nucleotide and predicted amino acid sequence analysis indicate that the carp NRAMP encodes a 548 amino acid membrane protein with 12 putative transmembrane domains, two N-Iinked glycosylation sites, and an evolutionarily conserved consensus transport motif. The peptide sequence identity among carp and human NRAMP2 is 78%, and 65% with human NRAMP1. Reverse transcription-polymerase chain reaction revealed that carp NRAMP is ubiquitously expressed. Phylogenetic analysis, using neigbor-joining, showed that the carp NRAMP protein clustered together with mammalian NRAMP2 proteins.

引用

页码：60 / 66

页数：7

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