Unnatural base pair systems toward the expansion of the genetic alphabet in the central dogma

被引：57

作者：

Hirao, Ichiro ^{[1
,2
]}

Kimoto, Michiko ^{[2
]}

机构：

[1] RIKEN, Syst & Struct Biol Ctr, Tsurumi Ku, Yokohama, Kanagawa 2300045, Japan

[2] TagCyx Biotechnol, Yokohama, Kanagawa, Japan

来源：

PROCEEDINGS OF THE JAPAN ACADEMY SERIES B-PHYSICAL AND BIOLOGICAL SCIENCES | 2012年 / 88卷 / 07期

关键词：

unnatural base pair; genetic alphabet; replication; transcription; translation; PCR; SITE-SPECIFIC INCORPORATION; DNA-REPLICATION FIDELITY; IN-VITRO TRANSCRIPTION; ENZYMATIC INCORPORATION; RNA MOLECULES; HYDROPHOBIC BASE; AMINO-ACID; SHAPE COMPLEMENTARITY; NUCLEOTIDE ANALOGS; DIRECTED EVOLUTION;

D O I：

10.2183/pjab.88.345

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Toward the expansion of the genetic alphabet of DNA, several artificial third base pairs (unnatural base pairs) have been created. Synthetic DNAs containing the unnatural base pairs can be amplified faithfully by PCR, along with the natural A-T and G-C pairs, and transcribed into RNA. The unnatural base pair systems now have high potential to open the door to next generation biotechnology. The creation of unnatural base pairs is a consequence of repeating "proof of concept" experiments. In the process, initially designed base pairs were modified to address their weak points. Some of them were artificially evolved to ones with higher efficiency and selectivity in polymerase reactions, while others were eliminated from the analysis. Here, we describe the process of unnatural base pair development, as well as the tests of their applications.

引用

页码：345 / 367

页数：23

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