Purification of the crude solution from Helix pomatia for use as β-glucuronidase and aryl sulfatase in phytoestrogen assays

被引：15

作者：

Grace, PB ^{[1
]}

Teale, P ^{[1
]}

机构：

[1] HFL, Fordham CB7 5WW, Cambs, England

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2006年 / 832卷 / 01期

关键词：

phytoestrogens; Isoflavones; lignans; Helix pomatia; glucuronidase; sulfatase; solid phase extraction; LC/MS/MS;

D O I：

10.1016/j.jchromb.2005.12.022

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Phytoestrogens occur in a variety of foods and are thought to offer a protective effect against a number of complex diseases. Due to the diversity of phytoestrogen conjugates formed in the human body, most assays include an enzymatic hydrolysis step prior to analysis. beta-Glucuronidase from Helix pomatia, which also contains sulfatase activity, is popular for this task but contains appreciable levels of some phytoestrogens and related compounds, which could affect accurate quantification at low concentrations. Use of solid phase extraction on a polymeric resin has been found to remove the majority of these compounds from the enzyme, without affecting the enzyme activity for almost all of the analytes tested. (c) 2005 Elsevier B.V. All rights reserved.

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收藏

页码：158 / 161

页数：4

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