Lower number and decreased function of natural killer cells in hepatitis B virus related acute-on-chronic liver failure

被引：22

作者：

Liu, Fangfang ^{[1
]}

Duan, Xuezhang ^{[2
]}

Wan, Zhihong ^{[2
]}

Zang, Hong ^{[2
]}

You, Shaoli ^{[2
]}

Yang, Ruichuang ^{[2
]}

Liu, Hongling ^{[2
]}

Li, Dongze ^{[2
]}

Li, Jin ^{[2
]}

Zhang, Yawei ^{[3
]}

Xin, Shaojie ^{[2
]}

机构：

[1] Chinese PLA Med Sch, Beijing, Peoples R China

[2] Beijing 302 Hosp, Beijing, Peoples R China

[3] Yale Univ, Yale Sch Publ Hlth, New Haven, CT USA

来源：

CLINICS AND RESEARCH IN HEPATOLOGY AND GASTROENTEROLOGY | 2016年 / 40卷 / 05期

关键词：

NK CELLS; HLA-C; RECEPTORS; KIR; EXPRESSION; INFECTION; LIGAND; EPIDEMIOLOGY; IMMUNE; LYSIS;

D O I：

10.1016/j.clinre.2016.01.004

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

Background: Hepatitis B virus (HBV)-related acute-on-chronic liver failure (ACLF) refers to acute deterioration occurring in patients with chronic hepatitis B infected liver diseases. An abnormality in NK cells mediated cellular immunity is believed to be a contributing factor. We aimed to evaluate the characteristic of NK cells in the peripheral blood of HBV related ACLF. Methods: Flow cytometric method was used to detect the absolute numbers and subgroups of NK cells, and analyze the cytotoxicity and killing ability of NK cells in patients with HBV-ACLF. Results: The results showed that peripheral numbers of NK cells were decreased in patients with HBV-ACLF, but not statistically significant. The cytotoxic CD56(dim)CD16(-) bright NK cells were significantly decreased in HBV infected patients, especially ACLF patients. The CD56(bright) CD16(-) subgroup was expanded in patients with CHB and the CD56(dim)CD16(-) subgroup was expanded in patients with ACLF. The activating receptors of NKG2D, NKp30, NKp44, and NKp46 were increased in patients with ACLF. The inhibitory receptors of CD158a were increased, though the CD158b was decreased in patients of ACLF. The function of NK cells including cytotoxicity and killing activity were both downregulated in patients with ACLF and CHB. Even if after IL12/15 stimulation, INF-gamma and TNF-alpha produced by patients with ACLF were still less than those produced by healthy controls. Conclusions: Patients with HBV-ACLF had lower numbers and decreased functions of cytotoxic NK cells. (C) 2016 Published by Elsevier Masson SAS.

引用

页码：605 / 613

页数：9

共 41 条

[1] Increased lipopolysaccharide binding protein in cirrhotic patients with marked immune and hemodynamic derangement [J].

Albillos, A ;

de la Hera, A ;

González, M ;

Moya, JL ;

Calleja, JL ;

Monserrat, J ;

Ruiz-del-Arbol, L ;

Alvarez-Mon, M .

HEPATOLOGY, 2003, 37 (01) :208-217

[2] Reduced frequencies of NKp30+NKp46+, CD161+, and NKG2D+NK cells in acute HCV infection may predict viral clearance [J].

Alter, Galit ;

Jost, Stephanie ;

Rihn, Suzannah ;

Reyor, Laura L. ;

Nolan, Brian E. ;

Ghebremichael, Musie ;

Bosch, Ronald ;

Altfeld, Marcus ;

Lauer, Georg M. .

JOURNAL OF HEPATOLOGY, 2011, 55 (02) :278-288

[3] Human natural killer cells [J].

Caligiuri, Michael A. .

BLOOD, 2008, 112 (03) :461-469

[4] NKp44, a triggering receptor involved in tumor cell lysis by activated human natural killer cells, is a novel member of the immunoglobulin superfamily [J].

Cantoni, C ;

Bottino, C ;

Vitale, M ;

Pessino, A ;

Augugliaro, R ;

Malaspina, A ;

Parolini, S ;

Moretta, L ;

Moretta, A ;

Biassoni, R .

JOURNAL OF EXPERIMENTAL MEDICINE, 1999, 189 (05) :787-795

[5]

Chinese Society of Hepatology and Chinese Society of Infectious Diseases Chinese Medical Association, 2011, Zhonghua Gan Zang Bing Za Zhi, V19, P13, DOI 10.3760/cma.j.issn.1007-3418.2011.01.007

[6] HLA-C IS THE INHIBITORY LIGAND THAT DETERMINES DOMINANT RESISTANCE TO LYSIS BY NK1-SPECIFIC AND NK2-SPECIFIC NATURAL-KILLER-CELLS [J].