12-oxo-phytodienoic acid and indole-3-acetic acid in jasmonic acid-treated tendrils of Bryonia dioica

Treatment of tendrils of Bryonia dioica with jasmonic acid (JA) or with the Pseudomonas toxin coronatine results in a severalfold and parallel increase in the levels of endogenous 12-oxo-phytodienoic acid (OPDA) and indole-3-acetic acid (IAA), both effective inducers of tendril coiling in this species. The dose-response curve far JA-induced OPDA reflects the biological activity of JA in inducing tendril coiling, suggesting that JA acts via endogenous OPDA A survey of a number of species most frequently used in JA research revealed that JA-induced increases of endogenous OPDA are not restricted to B. dioica, but do also occur in Nicotiana tabacum, Lepidium sativum, Hordeum vulgare and Brassica oleracea, but not in all species analyzed. The results reveal differences in the regulation of octadecanoid levels in different plant species and show that JA-treatment may have consequences on the auxin economy of a plant. In some species JA-treatment may affect the levels of OPDA, an octadecanoid that is itself biologically active. (C) 1999 Elsevier Science Ltd. All rights reserved.