High-performance liquid chromatographic determination of vancomycin in rabbit serum, vitreous and aqueous humour after intravitreal injection of the drug

被引：22

作者：

DelNozal, MJ ^{[1
]}

Bernal, JL ^{[1
]}

Pampliega, A ^{[1
]}

Marinero, P ^{[1
]}

Lopez, MI ^{[1
]}

Coco, R ^{[1
]}

机构：

[1] UNIV VALLADOLID,INST APPL OPHTHALMOBIOL,E-47005 VALLADOLID,SPAIN

来源：

JOURNAL OF CHROMATOGRAPHY A | 1996年 / 727卷 / 02期

关键词：

sample preparation; optimization; vancomycin; antibiotics;

D O I：

10.1016/0021-9673(95)01081-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A high-performance liquid chromatographic method for the determination of vancomycin in rabbit serum, vitreous and aqueous humour has been developed. No clean-up step was necessary for vitreous and aqueous humour samples. For serum samples liquid-liquid and solid-phase extraction were tested and the best results were achieved using C-18 cartridges. The extracts were analyzed on a C-18 reversed-phase column, using a mixture of 0.05 M phosphate buffer (pH 4) with 10% of acetonitrile as mobile phase. The detection was carried out at 198 nm, which allows higher sensitivity. The average quantitation limit obtained was 0.03 mu g/ml. The method has been applied to the study of the residual quantities of vancomycin in serum and rabbit eyes after intravitreal administration of the drug in endophthalmitis treatment.

引用

页码：231 / 238

页数：8

共 17 条

[1] COMPARISON OF RADIOIMMUNOASSAY AND FLUORESCENT POLARIZATION IMMUNOASSAY FOR QUANTITATIVE-DETERMINATION OF VANCOMYCIN CONCENTRATIONS IN SERUM [J].

ACKERMAN, BH ;

BERG, HG ;

STRATE, RG ;

ROTSCHAFER, JC .

JOURNAL OF CLINICAL MICROBIOLOGY, 1983, 18 (04) :994-995

[2] DETERMINATION OF VANCOMYCIN IN SERUM AND TISSUES BY COLUMN LIQUID-CHROMATOGRAPHY USING SOLID-PHASE EXTRACTION [J].

BAUCHET, J ;

PUSSARD, E ;

GARAUD, JJ .

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1987, 414 (02) :472-476

[3] VANCOMYCIN THERAPY OF EXPERIMENTAL PNEUMOCOCCAL MENINGITIS CAUSED BY PENICILLIN-SENSITIVE AND RESISTANT STRAINS [J].

BEAM, TR .

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 1981, 7 (01) :89-99

[4]

BORNER K, 1990, LABOR MED, V13, P270

[5]

GREENE SV, 1987, J CHROMATOGR, V47, P121

[6] RAPID AND SPECIFIC METHOD FOR THE DETERMINATION OF VANCOMYCIN IN PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY ON AN AMINOPROPYL COLUMN [J].

HOSOTSUBO, H .

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1989, 487 (02) :421-427

[7] MEASUREMENT OF VANCOMYCIN IN RENALLY IMPAIRED PATIENT SAMPLES USING A NEW HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY METHOD WITH VITAMIN-B12 INTERNAL STANDARD - COMPARISON OF HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY, EMIT, AND FLUORESCENCE POLARIZATION IMMUNOASSAY METHODS [J].

HU, MW ;

ANNE, L ;

FORNI, T ;

GOTTWALD, K .

THERAPEUTIC DRUG MONITORING, 1990, 12 (06) :562-569

[8] LEVELS OF VANCOMYCIN IN AQUEOUS-HUMOR AFTER TOPICAL EYE DROPS ADMINISTRATION [J].

HUERVA, V ;

SINUES, B ;

DELBUEY, MA ;

CRISTOBAL, JA ;

MINGUEZ, E ;

LANUZA, J ;

PALOMAR, A .

JOURNAL OF OCULAR PHARMACOLOGY, 1993, 9 (02) :167-170

[9] INACCURACIES DUE TO SAMPLE SOLVENT INTERACTIONS IN HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

INMAN, EL ;

MALONEY, AM ;

RICKARD, EC .

JOURNAL OF CHROMATOGRAPHY, 1989, 465 (02) :201-213

[10] MEASUREMENT OF ANTIMICROBIAL AGENTS IN CEREBROSPINAL-FLUID USING THE ABBOTT TDX ANALYZER [J].

JANDRESKI, MA ;

GARBINCIUS, J .

JOURNAL OF CLINICAL LABORATORY ANALYSIS, 1993, 7 (05) :263-268

← 1 2 →