Enhanced consistency in biological control of white grubs (Coleoptera: Scarabaeidae) with new strains of entomopathogenic nematodes

Achieving predictability (i.e., consistency of control) is a key challenge in the use of entomopathogenic nematodes for pest control. As nematode species/strain plays a key role in the success of white grub control, two strains, Heterorhabditis zealandica XI (Hz-X1) and Heterorhabditis bacteriophora GPS11 (Hb-GPS11), identified through laboratory assays to be the most virulent, were tested in a series of field trials against the second and third instar white grubs Popillia japonica and Cyclocephala borealis at different locations over a 2-year period. The HP88 strain of H. bacteriophora (Hb-HP88), the NJ and MB strains of Steinernema glaseri (Sg-NJ and Sg-MB) and a UK strain of Steinernema kraussei (Sk-UK) were also included for comparison in some tests. Overall, Hz-X1 and Hb-GPS11 strains were the most effective nematodes resulting in 73-98 and 34-97% control of P. japonica and 72-96 and 47-83% control of C borealis, respectively. The Hz-X1 and Hb-GPS11 strains did not differ significantly between each other against either grub species in any of the tests. The Hb-HP88 provided 52 and 36% control of P. japonica and C borealis, respectively. The Sg-MB provided 6-58% control of P. japonica, no control of C borealis, and 54-74% control of mixed populations of the two species. The Sg-NJ provided 20% control of P. japonica. Doubling the nematode application rate from 2.5 x 10(9) to 5 x 10(9) infective juveniles/ha did not significantly increase control of a mixed population of P. japonica and C borealis. Halofenozide (Mach 2 2SC and imidacloprid (Merit 75WP) applied as preventative treatments in July provided 97 and 98% control, respectively, of P. japonica. Imidacloprid applied as a curative treatment in September provided 47% control of C borealis but 0% control of P. japonica. Trichlorfon (Dylox 6.2G) applied as a curative treatment in September provided 29-92% control of P. japonica, 49% control of C borealis. and 0-77% control of mixed populations of the two grub species. Grub control provided by the Hz-X1 strain was always equal to or better than trichlorfon and that by the Hb-GPS11 strain was equal to trichlorfon except for one occasion. The amount of post-application irrigation plus rainfall had a strong effect on nematode efficacy. A total of 10 cm of post-application irrigation plus rainfall was found to be optimum at which the Hb-GPS11 and Hz-X1 strains produced 83-97 and 96-98% control of the two white grub species, respectively. Combined analyses of the results from all 8 trials containing 46 treatments indicate that the consistency in the control of P. japonica and C borealis by the new Hz-X1 and the Hb-GPS11 strains is equal to or better than the curative application of chemical insecticide standard, trichlorfon. (C) 2003 Elsevier Inc. All rights reserved.