Use of Flow Cytometry To Monitor Legionella Viability

被引：88

作者：

Allegra, Severine ^{[1
]}

Berger, Francoise ^{[1
]}

Berthelot, Philippe ^{[1
]}

Grattard, Florence ^{[1
]}

Pozzetto, Bruno ^{[1
]}

Riffard, Serge ^{[1
]}

机构：

[1] Univ St Etienne, Dept Genie Biol Genie Environm, IUT, Fac Med J Lisfranc,EA3064,GIMAP, F-42023 St Etienne 2, France

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2008年 / 74卷 / 24期

关键词：

D O I：

10.1128/AEM.01364-08

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Legionella viability was monitored during heat shock treatment at 70 C by a flow cytometric assay (FCA). After 30 min of treatment, for 6 of the 12 strains tested, the FCA still detected 10 to 25% of cells that were viable but nonculturable (VBNC). These VBNC cells were able to produce ATP and to be resuscitated after culture on amoebae.

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页码：7813 / 7816

页数：4

相关论文

共 29 条

[1]

*AFNOR, 1993, T90431 AFNOR NF

[2] Double-staining method for differentiation of morphological changes and membrane integrity of Campylobacter coli cells [J].

Alonso, JL ;

Mascellaro, S ;

Moreno, Y ;

Ferrús, MA ;

Hernández, J .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2002, 68 (10) :5151-5154

[3]

Armisen TG, 2004, WATER SCI TECHNOL, V50, P271

[4] Legionella:: from environmental habitats to disease pathology, detection and control [J].

Atlas, RM .

ENVIRONMENTAL MICROBIOLOGY, 1999, 1 (04) :283-293

[5] Bacterial viability and culturability [J].

Barer, MR ;

Harwood, CR .

ADVANCES IN MICROBIAL PHYSIOLOGY, VOL 41, 1999, 41 :93-137

[6] Assessment and interpretation of bacterial viability by using the LIVE/DEAD BacLight kit in combination with flow cytometry [J].

Berney, Michael ;

Hammes, Frederik ;

Bosshard, Franziska ;

Weilenmann, Hans-Ulrich ;

Egli, Thomas .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2007, 73 (10) :3283-3290

[7] Formation of nonculturable Escherichia coli in drinking water [J].

Bjergbæk, LA ;

Roslev, P .

JOURNAL OF APPLIED MICROBIOLOGY, 2005, 99 (05) :1090-1098

[8] Prospective 3-year surveillance for nosocomial and environmental Legionella pneumophila:: Implications for infection control [J].

Boccia, S. ;

Laurenti, P. ;

Borella, P. ;

Moscato, U. ;

Capalbo, G. ;

Cambieri, A. ;

Amore, R. ;

Quaranta, G. ;

Boninti, F. ;

Orsini, M. ;

Branca, G. ;

Fadda, G. ;

Spica, V. Romano ;

Ricciardi, G. .

INFECTION CONTROL AND HOSPITAL EPIDEMIOLOGY, 2006, 27 (05) :459-465

[9] LIVE/DEAD® BacLight™:: application of a new rapid staining method for direct enumeration of viable and total bacteria in drinking water [J].

Boulos, L ;

Prévost, M ;

Barbeau, B ;

Coallier, J ;

Desjardins, R .

JOURNAL OF MICROBIOLOGICAL METHODS, 1999, 37 (01) :77-86

[10]

*DGS, 2002, 2002243 DGSSD7ASD5CD, P243