Molecular Epidemiology and Phylogenetic Distribution of the Escherichia coli pks Genomic Island

被引:146
作者
Johnson, James R. [1 ,3 ]
Johnston, Brian [1 ,3 ]
Kuskowski, Michael A. [2 ,4 ]
Nougayrede, Jean-Philippe [5 ,6 ]
Oswald, Eric [5 ,6 ]
机构
[1] Vet Affairs Med Ctr, Med Serv, Minneapolis, MN 55417 USA
[2] Vet Affairs Med Ctr, Ctr Geriatr Res Educ & Clin, Minneapolis, MN 55417 USA
[3] Univ Minnesota, Dept Med, Minneapolis, MN 55455 USA
[4] Univ Minnesota, Dept Psychiat, Minneapolis, MN 55455 USA
[5] INRA, UMR 1225, F-31076 Toulouse, France
[6] Univ Toulouse, ENVT, UMR 1225, F-31076 Toulouse, France
基金
美国国家卫生研究院;
关键词
D O I
10.1128/JCM.00949-08
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Epidemiological and phylogenetic associations of the pks genomic island of extraintestinal pathogenic Escherichia coli (ExPEC), which encodes the genotoxin colibactin, are incompletely defined. clbB and clbN (as markers for the 5' and 3' regions of the pks island, respectively), clbA and clbQ (as supplemental pks island markers), and 12 other putative ExPEC virulence genes were newly sought by PCR among 131 published E. coli isolates from hospitalized veterans (62 blood isolates and 69 fecal isolates). Blood and fecal isolates and clbB-positive and -negative isolates were compared for 66 newly and previously assessed traits. Among the 14 newly sought traits, clbB and clbN (colibactin polyketide synthesis system), hra (heat-resistant agglutinin), and vat (vacuolating toxin) were significantly associated with bacteremia. clbB and clbN identified a subset within phylogenetic group B2 with extremely high virulence scores and a high proportion of blood isolates. However, by multivariable analysis, other traits were more predictive of blood source than clbB and clbN were; indeed, among the newly sought traits, only pic significantly predicted bacteremia (negative association). By correspondence analysis, clbB and clbN were closely associated with group B2 and multiple B2-associated traits; by principal coordinate analysis, clbB and clbN partitioned the data set better than did blood versus fecal source. Thus, the pks island was significantly associated with bacteremia, multiple ExPEC-associated virulence genes, and group B2, and within group B2, it identified an especially high-virulence subset. This extends previous work regarding the pks island and supports investigation of the colibactin system as a potential therapeutic target.
引用
收藏
页码:3906 / 3911
页数:6
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