Phase diagram of a 4-component lipid mixture: DSPC/DOPC/POPC/chol

被引：89

作者：

Konyakhina, Tatyana M. ^{[1
]}

Wu, Jing ^{[2
]}

Mastroianni, James D. ^{[1
]}

Heberle, Frederick A. ^{[3
]}

Feigenson, Gerald W. ^{[1
]}

机构：

[1] Cornell Univ, Dept Mol Biol & Genet, Ithaca, NY 14853 USA

[2] Univ Michigan, Dept Biophys, Ann Arbor, MI 48109 USA

[3] Oak Ridge Natl Lab, Biol & Soft Matter Div, Oak Ridge, TN 37831 USA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | 2013年 / 1828卷 / 09期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

4-Component lipid phase diagram; Quaternary phase diagram; Modulated phase; 3-Dye method; Competing interaction; Lipid raft; PLASMA-MEMBRANE VESICLES; MAXIMUM SOLUBILITY; MODEL BIOMEMBRANES; MODULATED PHASES; CHOLESTEROL; BILAYERS; BEHAVIOR; RAFTS; CONNECTIVITY; FLUCTUATIONS;

D O I：

10.1016/j.bbamem.2013.05.020

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report the first 4-component phase diagram for the lipid bilayer mixture, DSPC/DOPC/POPC/chol (distearoylphosphatidylcholine/dioleoylphosphatidylcholine/l-palmitoyl, 2-oleoylphosphatidylcholine/cholesterol). This phase diagram, which has macroscopic Ld + Lo phase domains, clearly shows that all phase boundaries determined for the 3-component mixture containing DOPC transition smoothly into the boundaries for the 3-component mixture containing POPC, which has nanoscopic phase domains of Ld + Lo. Our studies start from two published ternary phase diagrams, and show how these can be combined into a quaternary phase diagram by study of a few hundred samples of intermediate compositions. (C) 2013 Elsevier B.V. All rights reserved.

引用

页码：2204 / 2214

页数：11

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