Enzymatic synthesis of Kdn oligosaccharides by a bacterial α-(2→6)-sialyltransferase

被引：16

作者：

Kajihara, Y

Akai, S

Nakagawa, T

Sato, R

Ebata, T

Kodama, H

Sato, K

机构：

[1] Yokohama City Univ, Fac Sci, Dept Syst Funct, Kanazawa Ku, Yokohama, Kanagawa 2360027, Japan

[2] Kanagawa Univ, Fac Engn, Organ Chem Lab, Kanagawa Ku, Yokohama, Kanagawa 2218686, Japan

[3] Japan Tobacco Inc, Life Sci Res Lab, Midori Ku, Yokohama, Kanagawa 2278512, Japan

来源：

CARBOHYDRATE RESEARCH | 1999年 / 315卷 / 1-2期

关键词：

CMP-beta-D-Kdn; bacterial alpha-(2 -> 6)-sialyltransferase; enzymatic synthesis;

D O I：

10.1016/S0008-6215(98)00331-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Synthesis of CMP-deaminoneuraminic acid (CMP-beta-D-Kdn) and its enzymatic transfer reaction using bacterial alpha-(2 --> 6)-sialyltransferase were examined. CMP-beta-D-Kdn was prepared from methyl 4,5,7,8,9-penta-O-acetyl-3-deoxy-D-glycero-beta-D-galacto-2-nonulopyranosonate (2) in 24% overall yield. Enzymatic synthesis of Kdn oligosaccharide with CMP-beta-D-Kdn (10.2 mu mol), methyl beta-D-lactosaminide (7, 8.1 mu mol) and purified sialyltransferase (80 munits) afforded Kdn-alpha-(2 --> 6)-Gal-beta-(1 --> 4)-GlcNAc-beta-1-OMe in 77% yield. (C) 1999 Elsevier Science Ltd. All rights reserved.

引用

页码：137 / 141

页数：5

共 16 条

[1]

[Anonymous], 1994, ENZYMES SYNTHETIC OR

[2] AN EFFICIENT SYNTHESIS OF CYTIDINE MONOPHOSPHO-SIALIC ACIDS WITH 4 IMMOBILIZED ENZYMES [J].