Sex-related immune changes in young mice

被引:8
作者
Rosen, JL
Tran, HT
Lackey, A
Viselli, SM
机构
[1] Midwestern Univ, Dept Biochem, Downers Grove, IL 60515 USA
[2] Cytometry Associates, Brentwood, Essex, England
关键词
D O I
10.3109/08820139909060859
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Here we describe changes in selected immune parameters related to age and sex in young mice. We focused on the T cell compartment and studied thymuses and spleens from mice 3 to 9 weeks of age in order to bracket the time period around murine puberty. With regard to distribution of immune cells, no significant sex-related changes were seen in thymocyte expression of CD3, CD4, CD8, or CD4/CD8 or splenocyte expression of CD3, CD4, CD8, or CD45R/B220, a pan B cell marker. For splenocytes, significantly more cells were positive for CD3 in older (6-9 week old) compared with younger (3-4 week old) mice. Splenocyte and thymocyte cell proliferation as measured by DNA synthesis in response to in vitro mitogens was compared for cells from male and female mice over the ages studied. Thymocyte proliferation was not related to age or sex of the mice. For splenocytes of the youngest mice (3 weeks old), the response ts a cell surface-receptor-independent mitogenic combination of phorbol ester and ionomycin induced a significantly greater response in cells from female mice compared with male mice. This trend was reversed for mice of 4-6 weeks of age, where the response by splenocytes from males was significantly greater than that by cells from females. For mice 7-8 weeks of age, splenocytes from female mice responded significantly less to stimulation by antibody to CD3, a component of the T-cell receptor. Our results demonstrate that depending on the assays employed, sexual dimorphism in the immune system may be demonstrated prior to puberty.
引用
收藏
页码:247 / 256
页数:10
相关论文
共 28 条
[1]   SEASONAL DIFFERENCES IN THE RHYTHMICITY OF HUMAN MALE AND FEMALE LYMPHOCYTE BLASTOGENIC RESPONSES [J].
BOCTOR, FN ;
CHARMY, RA ;
COOPER, EL .
IMMUNOLOGICAL INVESTIGATIONS, 1989, 18 (06) :775-784
[2]   INFLUENCE OF SEX ON IMMUNOGLOBULIN LEVELS [J].
BUTTERWO.M ;
MCCLELLA.B ;
ALLANSMI.M .
NATURE, 1967, 214 (5094) :1224-&
[3]   Age-related changes in primary and secondary immune organs of the mouse [J].
Domínguez-Gerpe, L ;
Rey-Méndez, M .
IMMUNOLOGICAL INVESTIGATIONS, 1998, 27 (03) :153-165
[4]   STUDIES OF REGULATORY EFFECTS OF SEX-HORMONES ON ANTIBODY-FORMATION AND STEM CELL-DIFFERENTIATION [J].
EIDINGER, D ;
GARRETT, TJ .
JOURNAL OF EXPERIMENTAL MEDICINE, 1972, 136 (05) :1098-&
[5]   REAPPEARANCE OF THYMUS OF AGING RATS AFTER ORCHIDECTOMY [J].
FITZPATRICK, FTA ;
KENDALL, MD ;
WHEELER, MJ ;
ADCOCK, IM ;
GREENSTEIN, BD .
JOURNAL OF ENDOCRINOLOGY, 1985, 106 (03) :R17-R19
[6]   EPIDEMIOLOGIC ASPECTS OF HASHIMOTOS THYROIDITIS AND GRAVES-DISEASE IN ROCHESTER, MINNESOTA (1935-1967), WITH SPECIAL REFERENCE TO TEMPORAL TRENDS [J].
FURSZYFE.J ;
WOOLNER, LB ;
MCCONAHE.WM ;
ELVEBACK, LR ;
KURLAND, LT .
METABOLISM-CLINICAL AND EXPERIMENTAL, 1972, 21 (03) :197-+
[7]   REAPPEARANCE OF THE THYMUS IN OLD RATS AFTER ORCHIECTOMY - INHIBITION OF REGENERATION BY TESTOSTERONE [J].
GREENSTEIN, BD ;
FITZPATRICK, FTA ;
ADCOCK, IM ;
KENDALL, MD ;
WHEELER, MJ .
JOURNAL OF ENDOCRINOLOGY, 1986, 110 (03) :417-+
[8]  
Henderson J, 1904, J PHYSIOL-LONDON, V31, P222
[9]   UNDERSTANDING THE MECHANISM OF THE AGE-CHANGE OF THYMIC FUNCTION TO PROMOTE T-CELL DIFFERENTIATION [J].
HIROKAWA, K ;
UTSUYAMA, M ;
KASAI, M ;
KURASHIMA, C ;
ISHIJIMA, S ;
ZENG, YX .
IMMUNOLOGY LETTERS, 1994, 40 (03) :269-277
[10]   EPIDEMIOLOGY OF RHEUMATOID-ARTHRITIS - UPDATE [J].
HOCHBERG, MC ;
SPECTOR, TD .
EPIDEMIOLOGIC REVIEWS, 1990, 12 :247-252