The redox centers in the molybdo iron-sulfur flavoprotein CO dehydrogenase from the thermophilic carboxidotrophic bacterium Pseudomonas thermocarboxydovorans

被引：11

作者：

Hänzelmann, P ^{[1
]}

Hofmann, B ^{[1
]}

Meisen, S ^{[1
]}

Meyer, O ^{[1
]}

机构：

[1] Univ Bayreuth, Lehrstuhl Mikrobiol, D-95440 Bayreuth, Germany

来源：

FEMS MICROBIOLOGY LETTERS | 1999年 / 176卷 / 01期

关键词：

carbon monoxide; carboxidotrophic bacterium; CO dehydrogenase; molybdenum cofactor; molybdopterin-cytosine dinucleotide;

D O I：

10.1111/j.1574-6968.1999.tb13654.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The redox centers in the molybdo iron-sulfur flavoprotein CO dehydrogenase from the thermophilic bacterium Pseudomonas ther thermocarboxydovorans were identified and characterized by electron paramagnetic resonance (EPR). One mol of the 279-kDa dimer contained 1.9 mol of Mo, 2.2 mol of FAD, 6.9 mol of Fe and 6.7 mol of labile sulfide. The molybdenum cofactor is composed of a 1:1 mononuclear complex of molybdopterin-cytosine dinucleotide and the Mo ion. EPR spectroscopy revealed signals typical for Mo(V), FADH(.), and type I and type II [2Fe-2S] centers. (C) 1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：139 / 145

页数：7

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