Met-enkephalin induces cytolytic processes of apoptotic type in K562 human erythroid leukemia cells

被引：19

作者：

Mernenko, OA ^{[1
]}

Blishchenko, EY ^{[1
]}

Mirkina, II ^{[1
]}

Karelin, AA ^{[1
]}

机构：

[1] RUSSIAN ACAD SCI,SHEMYAKIN OVCHINNIKOV INST BIOORGAN CHEM,MOSCOW 117871,RUSSIA

来源：

FEBS LETTERS | 1996年 / 383卷 / 03期

关键词：

Met-enkephalin; cytotoxicity; apoptosis;

D O I：

10.1016/0014-5793(96)00208-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cytolytic activity of Met-enkephalin, an endogenous opioid peptide, was studied within the 10(-7)-10(-17) M concentration range in K562 human erythroid leukemia cells. Cytolytic activity was determined by the trypan blue inclusion method after 13, 15 and 18 h of Met-enkephalin co-incubation with target cells. Discrete maxima of cytolytic activity were detected at concentrations of 10(-9)-10(-10), 10(-13) and 10(-15) M. Cytolysis was accompanied by internucleosomal DNA fragmentation which is indicative of the mechanism of cell death being apoptosis.

引用

页码：230 / 232

页数：3

共 20 条

[1]

BLISHCHENKO E, 1995, J CELL BIOCH B S, V19, P200

[2] TUBOCURARIN INDUCES THE WIDE SPECTRUM OF CYTOLYTIC EFFECTS IN TUMOR-CELLS [J].

BLISHCHENKO, EY ;

KARELIN, AA .

IMMUNOLOGY LETTERS, 1994, 42 (1-2) :13-17

[3] FAS AND TUMOR-NECROSIS-FACTOR RECEPTOR-MEDIATED CELL-DEATH - SIMILARITIES AND DISTINCTIONS [J].

CLEMENT, MV ;

STAMENKOVIC, I .

JOURNAL OF EXPERIMENTAL MEDICINE, 1994, 180 (02) :557-567

[4] CELL-DEATH MECHANISMS AND THE IMMUNE-SYSTEM [J].

GOLSTEIN, P ;

OJCIUS, DM ;

YOUNG, JDE .

IMMUNOLOGICAL REVIEWS, 1991, 121 :29-65

[5]

GUSTIN T, 1993, CANCER RES, V53, P4715

[6]

LINNER KM, 1995, J IMMUNOL, V154, P5049

[7]

MANECKJEE R, 1994, CELL GROWTH DIFFER, V5, P1033

[8]

MURGO AJ, 1985, J NATL CANCER I, V75, P341

[9] IONOPHORE-INDUCED APOPTOSIS - ROLE OF DNA FRAGMENTATION AND CALCIUM FLUXES [J].

OJCIUS, DM ;

ZYCHLINSKY, A ;

LI, MZ ;

YOUNG, JDE .

EXPERIMENTAL CELL RESEARCH, 1991, 197 (01) :43-49

[10] ENDOGENOUS OPIATES - 1992 [J].

OLSON, GA ;

OLSON, RD ;

KASTIN, AJ .

PEPTIDES, 1993, 14 (06) :1339-1378

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