Identification of an insertion sequence, IS1203 variant, in a Shiga toxin 2 gene of Escherichia coli O157:H7

被引：27

作者：

Kusumoto, M ^{[1
]}

Nishiya, Y

Kawamura, Y

Shinagawa, K

机构：

[1] Toyobo Co Ltd, Tsuruga Inst Biotechnol, Fukui 9140047, Japan

[2] Iwate Univ, Dept Vet Med, Morioka, Iwate 0208550, Japan

来源：

JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 1999年 / 87卷 / 01期

关键词：

insertion sequence; Shiga toxin gene; Escherichia coli O157 : H7;

D O I：

10.1016/S1389-1723(99)80014-0

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

An insertion sequence composed of 1310 bp was found in Shiga toxin 2 genes of some isolates of Escherichia coli O157:H7. This insertion sequence showed extremely high homology with IS1203 of E. coli O111:H-. This IS1203 variant was inserted in the region encoding the amino-terminus of the B subunit with a duplication of 3 bp at the target site, resulting in inactivation of the Shiga toxin 2 gene.

引用

页码：93 / 96

页数：4

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