An Epigenetic Role for Maternally Inherited piRNAs in Transposon Silencing

被引:554
作者
Brennecke, Julius [1 ]
Malone, Colin D. [1 ]
Aravin, Alexei A. [1 ]
Sachidanandam, Ravi [1 ]
Stark, Alexander [2 ,3 ,4 ]
Hannon, Gregory J. [1 ]
机构
[1] Cold Spring Harbor Lab, Howard Hughes Med Inst, Watson Sch Biol Sci, Cold Spring Harbor, NY 11724 USA
[2] MIT, Broad Inst, Cambridge, MA 02141 USA
[3] Harvard Univ, Broad Inst, Cambridge, MA 02141 USA
[4] MIT, Comp Sci & Artificial Intelligence Lab, Cambridge, MA 02139 USA
基金
奥地利科学基金会;
关键词
D O I
10.1126/science.1165171
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
In plants and mammals, small RNAs indirectly mediate epigenetic inheritance by specifying cytosine methylation. We found that small RNAs themselves serve as vectors for epigenetic information. Crosses between Drosophila strains that differ in the presence of a particular transposon can produce sterile progeny, a phenomenon called hybrid dysgenesis. This phenotype manifests itself only if the transposon is paternally inherited, suggesting maternal transmission of a factor that maintains fertility. In both P- and I- element- mediated hybrid dysgenesis models, daughters show a markedly different content of Piwi- interacting RNAs ( piRNAs) targeting each element, depending on their parents of origin. Such differences persist from fertilization through adulthood. This indicates that maternally deposited piRNAs are important for mounting an effective silencing response and that a lack of maternal piRNA inheritance underlies hybrid dysgenesis.
引用
收藏
页码:1387 / 1392
页数:6
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