An assay for the quantitation of Photofrin(R) in tissues and fluids

被引：33

作者：

Bellnier, DA

Greco, WR

Parsons, JC

Oseroff, AR

Kuebler, A

Dougherty, TJ

机构：

[1] ROSWELL PK CANC INST, DEPT BIOMATH, BUFFALO, NY 14263 USA

[2] ROSWELL PK CANC INST, DEPT DERMATOL, BUFFALO, NY 14263 USA

[3] UNIV HEIDELBERG, DEPT ORAL & MAXILLOFACIAL SURG, HEIDELBERG, GERMANY

来源：

PHOTOCHEMISTRY AND PHOTOBIOLOGY | 1997年 / 66卷 / 02期

关键词：

D O I：

10.1111/j.1751-1097.1997.tb08649.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for determining the concentration of Photofrin(R) in tissues and biological fluids was developed. The procedure is based on the dissolution of biological material with Solvable(R), a commercially available tissue solubilizer, followed by porphyrin-specific fluorescence detection and measurement. It was found necessary to use a quadratic standard curve for the estimation of unknown Photofrin(R) concentrations. While this method is limited to compounds that are stable in strong base, it has the advantages of being sensitive, rapid and low cost.

引用

页码：237 / 244

页数：8

共 17 条

[1]

Bellnier D A, 1996, J Clin Laser Med Surg, V14, P311

[2] DISTRIBUTION AND ELIMINATION OF PHOTOFRIN-II IN MICE [J].

BELLNIER, DA ;

HO, YK ;

PANDEY, RK ;

MISSERT, JR ;

DOUGHERTY, TJ .

PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1989, 50 (02) :221-228

[3]

Brasseur N, 1995, PHOTOCHEM PHOTOBIOL, V62, P1058

[4]

BROWN SB, 1992, INT CONGR SER, V1011, P475

[5]

DIXON WJ, 1990, BMDP STATISTICAL SOF, V1

[6]

DIXON WJ, 1990, BMDP STATISTICAL SOF, V2

[7]

DRAPER NR, 1981, APPLIED REGRESSION A

[8]

Fox E., 1995, SIGMASTAT STAT SOFTW

[9]

GOMER CJ, 1979, CANCER RES, V39, P146

[10] PHOTOSENSITIZATION OF MURINE TUMOR, VASCULATURE AND SKIN BY 5-AMINOLEVULINIC ACID-INDUCED PORPHYRIN [J].

HENDERSON, BW ;

VAUGHAN, L ;

BELLNIER, DA ;

VANLEENGOED, H ;

JOHNSON, PG ;

OSEROFF, AR .

PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1995, 62 (04) :780-789

← 1 2 →