The polycystic ovarian (PCO) condition: Apoptosis and epithelialization of the ovarian antral follicles are aspects of cystogenesis in the dehydroepiandrosterone (DHEA)-treated rat model

被引:36
作者
Anderson, E
Lee, GY
机构
[1] Department of Cell Biology, Harvard Medical School, Boston
关键词
polycystic ovary; cystogenesis; hyperandrogenism; apoptosis; epithelialization;
D O I
10.1016/S0040-8166(97)80017-1
中图分类号
R602 [外科病理学、解剖学]; R32 [人体形态学];
学科分类号
100101 ;
摘要
This investigation was designed to study apoptosis and epithelialization during cystogenesis of the dehydroepiandrosterone rat model, Using in situ DNA 3'- end-labeling with non-radioactive digoxigenin-didesoxy-UTP (dig-ddUTP), apoptosis is initially seen in cumulus granulosa cells and other granulosa cells facing the antrum, During cystogenesis, apoptosis systematically progresses from the cumulus towards the mural granulosa layer. In contrast, granulosa cells of atretic follicles undergo apoptosis in a random manner, The outer layer of mural granulosa cells during cystogenesis escapes apoptosis. Granulosa cells contain vimentin. However, the outer mural granulosa cell layer that lines the cyst acquires keratin. In addition to being associated with each other via gap junctions, the outer layer of granulosa cells acquire tight junctions. With the characterization of the transformation of the outer mural granulosa cells into a characteristic epithelium and the orderly progression of apoptosis, we further the understanding of the multifaceted process of cystogenesis of the ovarian antral follicle.
引用
收藏
页码:171 / 189
页数:19
相关论文
共 40 条
[1]   APPEARANCE AND STRUCTURE OF INTERCELLULAR CONNECTIONS DURING ONTOGENY OF RABBIT OVARIAN FOLLICLE WITH PARTICULAR REFERENCE TO GAP JUNCTIONS [J].
ALBERTINI, DF ;
ANDERSON, E .
JOURNAL OF CELL BIOLOGY, 1974, 63 (01) :234-250
[2]   CYTOLOGICAL OBSERVATIONS ON THE FINE STRUCTURE OF THE GUINEA PIG OVARY WITH SPECIAL REFERENCE TO THE OOGONIUM, PRIMARY OOCYTE AND ASSOCIATED FOLLICLE CELLS [J].
ANDERSON, E ;
BEAMS, HW .
JOURNAL OF ULTRASTRUCTURE RESEARCH, 1960, 3 (04) :432-446
[3]   CYSTOGENESIS OF THE OVARIAN ANTRAL FOLLICLE OF THE RAT - ULTRASTRUCTURAL-CHANGES AND HORMONAL PROFILE FOLLOWING THE ADMINISTRATION OF DEHYDROEPIANDROSTERONE [J].
ANDERSON, E ;
LEE, MT ;
LEE, GY .
ANATOMICAL RECORD, 1992, 234 (03) :359-382
[4]   AN INVITRO STUDY OF THE EFFECTS OF ANDROGENS ON THE CYTOSKELETON OF OVARIAN GRANULOSA-CELLS WITH SPECIAL REFERENCE TO ACTIN [J].
ANDERSON, E ;
SELIG, M ;
LEE, GY ;
LITTLE, B .
TISSUE & CELL, 1988, 20 (06) :855-874
[5]  
Anderson E., 1995, Molecular Biology of the Cell, V6, p425A
[6]   PRODUCTION OF ANOVULATORY, STERILE RATS BY SINGLE INJECTIONS OF TESTOSTERONE PROPIONATE [J].
BARRACLOUGH, C .
ENDOCRINOLOGY, 1961, 68 (01) :62-&
[7]   THE CYTOPLASMIC FILAMENTOUS NETWORK IN CULTURED OVARIAN GRANULOSA-CELLS [J].
BATTEN, BE ;
AALBERG, JJ ;
ANDERSON, E .
CELL, 1980, 21 (03) :885-895
[8]  
CZERNOBILSKY B, 1985, EUR J CELL BIOL, V37, P175
[9]  
Eppig John J., 1994, Seminars in Developmental Biology, V5, P51, DOI 10.1006/sedb.1994.1007
[10]   A 24-HOUR PERIODICITY IN THE LH-RELEASE APPARATUS OF FEMALE RATS, DISCLOSED BY BARBITURATE SEDATION [J].
EVERETT, JW ;
SAWYER, CH .
ENDOCRINOLOGY, 1950, 47 (03) :198-218