Characterisation of Triticum vavilovii-derived stripe rust resistance using genetic, cytogenetic and molecular analyses and its marker-assisted selection

被引:46
作者
Bariana, HS
Brown, GN
Ahmed, NU
Khatkar, S
Conner, RL
Wellings, CR
Haley, S
Sharp, PJ
Laroche, A
机构
[1] Univ Sydney, Plant Breeding Inst Cobbity, Camden, NSW 2570, Australia
[2] Agr & Agr Food Canada Res Ctr, Lethbridge, AB, Canada
[3] Colorado State Univ, Ft Collins, CO 80523 USA
关键词
stripe rust; chromosome location; microsatellite; gliadin; glume colour; marker-assisted selection;
D O I
10.1007/s001220100767
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Stripe rust resistance was identified in Triticum vavilovii (T. vavilovii Aus22498)-derived Russian wheat aphid (RWA)-resistant germplasm. Inheritance studies indicated monogenic control of resistance. The resistance gene was tentatively designated as Yrvav and was located on chromosome 1 B by monosomic analysis. A close association (1.5+/-0.9% recombination) of Yrvav with a T. vavilovii-derived gliadin allele (Gli-B1vav) placed it in chromosome arm 1BS. Yrvav was allelic with Yr10. Tests with Yr10 avirulent and virulent pathotypes showed that Yrvav and Yr10 possess identical pathogenic specificity. Yrvav and Yr10 showed close genetic associations with alternate alleles at the Xpsp3000 (microsatellite marker), Gli-B1 and Rg1 loci. Based on these observations Yrvav was named as Yr10vav. The close association between Xpsp3000 and Gli-B1 was also confirmed. The Yr10vav-linked Xpsp3000 allele (285 bp) was not present in 65 Australian cultivars, whereas seven Australian wheats lacking Yr10 carried the same Xpsp3000 allele (260 bp) as Yr10 carrying wheat cultivar Moro. Xpsp3000 and/or Gli-B1 could be used in marker-assisted selection for pyramiding Yr10vav or Yr10 with other stripe rust resistance genes. Yr10vav was inherited independently of the T. vavilovii-derived RWA resistance.
引用
收藏
页码:315 / 320
页数:6
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