The effect of hypoxia on lipid phosphate receptor and sphingosine kinase expression and mitogen-activated protein kinase signaling in human pulmonary smooth muscle cells

被引：45

作者：

Ahmad, Maqsood ^{[1
]}

Long, Jaclyn S. ^{[1
]}

Pyne, Nigel J. ^{[1
]}

Pyne, Susan ^{[1
]}

机构：

[1] Univ Strathclyde, Strathclyde Inst Biomed Sci, Dept Physiol & Pharmacol, Glasgow G4 0NR, Lanark, Scotland

来源：

PROSTAGLANDINS & OTHER LIPID MEDIATORS | 2006年 / 79卷 / 3-4期

关键词：

sphingosine 1-phosphate receptor; lysophosphatidic acid receptor; sphingosine kinase; lipid phosphate phosphatase; extracellular signal regulated kinase;

D O I：

10.1016/j.prostaglandins.2006.03.001

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Both acute and chronic hypoxia had no effect on S1P(1), S1P(3) or LPA(1) receptor transcript expression inhuman pulmonary smooth muscle cells. However, acute hypoxia increased sphingosine kinase SK1/2 and LPP1 mRNA transcript levels, while chronic hypoxia increased SKI mRNA transcript alone. Acute hypoxia had no effect on S1P-, PDGF- or phorbol ester (PMA)-stimulated activation of ERK-1/2, but increased the ability of SIP to activate p38 MAPK. Chronic hypoxia increased the ability of SIP to stimulate the phosphorylation of ERK-1/2. Therefore, we have demonstrated for the first time that hypoxia can lead to marked changes in the expression of genes involved in SIP production and may modify post SIP receptor signal transduction pathways. (c) 2006 Elsevier Inc. All rights reserved.

引用

页码：278 / 286

页数：9

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