Outward potassium currents of supraoptic magnocellular neurosecretory cells isolated from the adult guinea-pig

1. Several types of whole-cell outward K+ current recorded from magnocellular neurosecretory cells (MNCs) dissociated from the supraoptic nucleus of the adult guinea-pig were identified on the basis of their voltage dependence, kinetics, pharmacology and Ca2+ dependence. 2. The predominant K+ current evoked from a holding potential of -40 mV was slowly activating, long-lasting, tetraethylammonium (TEA) sensitive and showed little steady-state inactivation. Also, this current was reduced by extracellular Cd2+. These data suggest that in supraoptic MNCs classical Ca2+-insensitive, delayed rectifier channels (K-v) and Ca2+-sensitive, non-inactivating channels (K-Ca) both contribute to the sustained current. 3. A transient, low-threshold K+ current, which was 4-aminopyridine (4-AP) sensitive and showed significant steady-state inactivation, was evoked dong with the sustained current from a holding potential of -90 mV. Based on these characteristics, this current corresponds to the A-current (I-K(A)) described in other neurons. 4. I-K(A) was activated when Ca2+ influx was blocked or when Ca2+ was absent from the extracellular medium, suggesting that Ca2+ influx is not necessary for activation of the current. 5. In many recordings, a transient 4-AP-insensitive outward current was evoked from a holding potential of -40 mV. This high-threshold transient K+ current was abolished by extracellular Cd2+ or TEA and was absent when extracellular Ca2+ was replaced by Sr2+ suggesting that it is a transient Ca2+-dependent K+ current. 6. We conclude that the presence of multiple types of K+ current may, in part, underlie the complex firing patterns of oxytocinergic and vasopressinergic MNCs.