Kinetic studies on elemental sulfur oxidation by Acidithiobacillus ferrooxidans:: Sulfur limitation and activity of free and adsorbed bacteria

被引：35

作者：

Ceskova, P ^{[1
]}

Mandl, M ^{[1
]}

Helanova, S ^{[1
]}

Kasparovska, J ^{[1
]}

机构：

[1] Masaryk Univ, Fac Sci, Dept Biochem, CS-61137 Brno, Czech Republic

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 2002年 / 78卷 / 01期

关键词：

Acidithiobacillus ferrooxidans; Thiobacillus; sulfur oxidation; growth kinetics; adsorption of bacteria;

D O I：

10.1002/bit.10172

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The kinetics of sulfur oxidation by Acidithio-bacillus ferrooxidans in shaking flasks and a 10-L reactor was studied. The observed linearity of growth and sulfur oxidation was explained by sulfur limitation. Total cell yield was not significantly different for exponential growth as compared to growth during the sulfur-limiting phase. Kinetic studies of sulfur oxidation by growing and nongrowing bacteria indicated that both free and adsorbed bacteria oxidize sulfur. Changes in the number of free bacteria rather than cells adsorbed on sulfur were better predictors of the kinetics of sulfur oxidation, indicating that the free bacteria were performing sulfur oxidation. The active growth phase always followed adsorption of bacteria on sulfur; however, the special metabolic role of adsorbed bacteria was unclear. Their activity in sulfur solubilization was considered. (C) 2002 Wiley Periodicals, Inc.

引用

页码：24 / 30

页数：7

共 15 条

[1]

Barret J., 1993, METAL EXTRACTION BAC

[2] Bioleaching: Metal solubilization by microorganisms [J].

Bosecker, K .

FEMS MICROBIOLOGY REVIEWS, 1997, 20 (3-4) :591-604

[3] Kinetic quantitation of sulfur-oxidizing bacteria adsorbed on sulfur [J].

Cesková, P ;

Mandl, M ;

Hubácková, J .

BIOTECHNOLOGY LETTERS, 2000, 22 (08) :699-701

[4] Mechanism and kinetics of elemental sulfur oxidation by Thiobacillus thiooxidans in batch fermenter [J].

Cheng, YC ;

Peng, RY ;

Su, JCC ;

Lo, DY .

ENVIRONMENTAL TECHNOLOGY, 1999, 20 (09) :933-942

[5] GROWTH OF THIOBACILLUS-FERROOXIDANS ON ELEMENTAL SULFUR [J].

ESPEJO, RT ;

ROMERO, P .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1987, 53 (08) :1907-1912

[6]

Gourdon R, 1998, BIOPROCESS ENG, V18, P241

[7]

HANSFORD GS, 1997, BIOMINING THEORY MIC, P151

[8] Metabolic activity of Thiobacillus ferrooxidans on reduced sulfur compounds detected by capillary isotachophoresis [J].

Janiczek, O ;

Mandl, M ;

Cesková, P .

JOURNAL OF BIOTECHNOLOGY, 1998, 61 (03) :225-229

[9] KINETICS OF GROWTH AND ELEMENTAL SULFUR OXIDATION IN BATCH CULTURE OF THIOBACILLUS-FERROOXIDANS [J].

KONISHI, Y ;

TAKASAKA, Y ;

ASAI, S .

BIOTECHNOLOGY AND BIOENGINEERING, 1994, 44 (06) :667-673

[10] GROWTH-KINETICS OF THIOBACILLUS THIOOXIDANS ON THE SURFACE OF ELEMENTAL SULFUR [J].

KONISHI, Y ;

ASAI, S ;

YOSHIDA, N .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1995, 61 (10) :3617-3622

← 1 2 →