MicroRNA 363 mediated positive regulation of c-myc translation affect prostate cancer development and progress

被引：24

作者：

Chen, Y. ^{[1
,2
]}

Lu, X. ^{[2
]}

Wu, B. ^{[2
]}

Su, Y. ^{[2
]}

Li, J. ^{[2
]}

Wang, H. ^{[1
]}

机构：

[1] Fourth Mil Med Univ, Tang Du Hosp, Dept Urol, Xian 710038, Shannxi Provinc, Peoples R China

[2] PLA, Hosp 323, Dept Urol, Xian 710054, Shannxi Provinc, Peoples R China

来源：

NEOPLASMA | 2015年 / 62卷 / 02期

关键词：

prostate cancer; transformation property; proliferation; micro-363; epithelial-to-mesenchymal transition; EXPRESSION; CELLS; METASTASIS; APOPTOSIS;

D O I：

10.4149/neo_2015_024

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 [肿瘤学];

摘要：

Prostate cancer (CaP) is the sixth most significant cancer killer of men in China. In this study, the potential role of micro-363 (miR-363) in CaP development and progression was investigated. Pri-miR-363 or anti-miR-363 was transfected into the CaP cells line PC-3 cells. Cell proliferation, transformation property, and epithelial-to-mesenchymal transition (EMT) were evaluated by MTT, clonogenic assay, colony formation in soft agar and western blotting, respectively. The expression and involvement of c-myc, a downstream target of miR-363 were also determined. The results showed that endogenous expression of miR-363 was significantly increased in CaP cells compared with normal prostate cells. High expression of miR-363 in PC-3 cells through transfection induces cell proliferation and positively regulates cell transformation property as well as promotes EMT of PC-3 cells. Through knockdown of c-myc, the results also showed that c-myc was involved in the regulation of biological function of PC-3 cells by miR-363. Taken together, this study adds support to the potential role of miR-363 in the diagnosis and treatment of CaP.

引用

页码：191 / 198

页数：8

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