Chemical and functional studies on the importance of purple membrane lipids in bacteriorhodopsin photocycle behavior

被引：45

作者：

Dracheva, S ^{[1
]}

Bose, S ^{[1
]}

Hendler, RW ^{[1
]}

机构：

[1] NHLBI,NIH,CELL BIOL LAB,BETHESDA,MD 20892

来源：

FEBS LETTERS | 1996年 / 382卷 / 1-2期

关键词：

bacteriorhodopsin photocycle; membrane lipid; membrane protein; photocycle regulation;

D O I：

10.1016/0014-5793(96)00181-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In native purple membrane (PM), there are approximately 1 squalene, 2 glycolipid sulfate (GLS), and 6 phospholipid (PL) molecules per bacteriorhodopsin (BR) monomer [10], Brief (similar to 2 min) exposure to 0.1% Triton X-100 removes about 25%, 20%, and 6% of squalenes, GLS, and FL, respectively (this paper) while causing profound changes in the BR photocycle, including the loss of 'photocooperativity' [1]. The BR photocycle in Triton-treated PM can be restored to near normal behavior by reconstitution with native PM lipids, Isolated squalenes are not effective whereas PL alone partially restores normal photocycle characteristics.

引用

页码：209 / 212

页数：4

共 11 条

[1]

[Anonymous], 2009, FEMS MICROBIOL LETT, DOI DOI 10.1111/j.1574-6968.2009.01638.x

[2]

BLIGH EG, 1959, CAN J BIOCHEM PHYS, V37, P911

[3]

DRACHEV LA, 1993, BIOCHEM MOL BIOL INT, V30, P461

[4] RESONANCE RAMAN AND OPTICAL TRANSIENT STUDIES ON THE LIGHT-INDUCED PROTON PUMP OF BACTERIORHODOPSIN REVEAL PARALLEL PHOTOCYCLES [J].