High-resolution 1H NMR spectroscopy in rat liver using magic angle turning at a 1 Hz spinning rate

It is demonstrated that a high-resolution H-1 NMR spectrum of excised rat liver can be obtained using the technique of magic angle turning (MAT) at a sample spinning rate of 1 Hz. A variant of the phase-corrected MAT (PHORMAT) pulse sequence that includes a water suppression segment was developed for the investigation. The spectral resolution achieved with PHORMAT approaches that obtained from a standard magic angle spinning (MAS) experiment at a spinning rate of several kHz. With such ultra-slow spinning, tissue and cell damage associated with the standard MAS experiment is minimized or eliminated. The technique is potentially useful for obtaining highresolution H-1 spectra in live animals. (C) 2002 Wiley-Liss, Inc.