A cyanobacterial narB gene encodes a ferredoxin-dependent nitrate reductase

被引：46

作者：

Rubio, LM ^{[1
]}

Herrero, A ^{[1
]}

Flores, E ^{[1
]}

机构：

[1] UNIV SEVILLA,CSIC,INST BIOQUIM VEGETAL & FOTOSINTESIS,E-41080 SEVILLE,SPAIN

来源：

PLANT MOLECULAR BIOLOGY | 1996年 / 30卷 / 04期

关键词：

nitrate reductase; Synechococcus;

D O I：

10.1007/BF00019017

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The narB gene from the cyanobacterium Synechococcus sp. PCC 7942 was cloned downstream from the LacI-regulated promoter P-trc in the Escherichia coli vector pTrc99A, rendering plasmid pCSLM1. Addition of isopropyl-beta-D-thiogalactoside to E. coli (pCSLM1) resulted in the parallel expression of a 76 kDa polypeptide and a nitrate reductase activity with properties identical to those known for nitrate reductase isolated from Synechococcus cells. As is the case for nitrate reductase from Synechococcus cells, either reduced methyl viologen or reduced ferredoxin could be used as an electron donor for the reduction of nitrate catalyzed by E. coli (pCSLM1) extracts. This data shows that narB is a cyanobacterial structural gene for nitrate reductase.

引用

页码：845 / 850

页数：6

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