Molecular characterization of a cDNA from Pseudaletia unipuncta encoding the Manduca sexta allatostatin peptide (Mas-AST)

被引:70
作者
Jansons, IS
Cusson, M
McNeil, JN
Tobe, SS
Bendena, WG
机构
[1] QUEENS UNIV,DEPT BIOL,KINGSTON,ON K7L 3N6,CANADA
[2] LAURENTIAN FORESTRY CTR,ST FOY,PQ G1V 4C7,CANADA
[3] UNIV LAVAL,DEPT BIOL,ST FOY,PQ G1K 7P4,CANADA
[4] UNIV TORONTO,DEPT ZOOL,TORONTO,ON M5S 1A1,CANADA
关键词
juvenile hormone; proteolytic processing; neurobiology; lepidoptera;
D O I
10.1016/S0965-1748(96)00023-9
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A 15-residue neuropeptide, Manduca sexta allatostatin (Mas-AST), strongly inhibits juvenile hormone (JH) biosynthesis in vitro by corpora allata (CA) from Manduca fifth-stadium larvae and adult females as well as Helicoverpa tea adult females (Kramer et al., 1991, Proc, Natl, Acad, Sci (USA) 88, 9458-9462), In contrast, this study found that 1.0 mu M Mas-AST has no JH biosynthesis inhibitory activity in Pseudaletia unipuncta sixth instar larvae or newly-emerged (day 0) adults but inhibited CA of 5-day-old adult females by 60%, From a P. unipuncta brain cDNA library, was isolated a cDNA that encodes a 125 amino acid polypeptide containing the Mas-AST sequence, Within the precursor, Mas-AST is situated at the carboxy terminus and is flanked by different dibasic proteolytic cleavage signals, The Pseudaletia gene specifying the Mas-AST peptide is present as a single copy per haploid genome, Expression of this gene was low in Pseudaletia sixth instar larvae, prepupae and early pupae but was relatively high in late pupae, and day 1 and 3 adults of both sexes, In day 5 adults, the relative transcript level appears to be maintained in females but declines in males, This pattern of Mas-AST expression does not correlate well with the profile of JH biosynthesis in Pseudaletia, which increases during the first 5 days of adult life, suggesting additional or alternative functions for this peptide. Copyright (C) 1996 Elsevier Science Ltd
引用
收藏
页码:767 / 773
页数:7
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