Detection of porcine circovirus types 1 and 2 in serum and tissue samples of pigs with and without postweaning multisystemic wasting syndrome

被引:71
作者
Calsamiglia, M
Segalés, J
Quintana, J
Rosell, C
Domingo, M
机构
[1] Univ Autonoma Barcelona, Fac Vet, Dept Sanitat & Anat Anim, E-08193 Barcelona, Spain
[2] Ctr Reccerca Sanitat Anim, E-08193 Barcelona, Spain
关键词
D O I
10.1128/JCM.40.5.1848-1850.2002
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Presence of porcine circovirus type 1 (PCV1) and PCV2 was studied in sera and superficial inguinal lymph nodes from postweaning multisystemic wasting syndrome (PMWS)-affected and non-PMWS-affected pigs by using in situ hybridization and PCR. PCV1 and PCV2 were found in less than 3% and more than 50% of the samples, respectively. The most sensitive technique and site was PCR in superficial inguinal lymph nodes, but in situ hybridization correlated better with presence of characteristic lesions.
引用
收藏
页码:1848 / 1850
页数:3
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